中国媒介生物学及控制杂志 ›› 2010, Vol. 21 ›› Issue (1): 30-32.

• 论著 • 上一篇    下一篇

冷冻丝光绿蝇蛹对丽蝇蛹集金小蜂后代发育和繁殖能力的影响

张忠,宋光乐,于广福,庄东明,万文菊,赵英会,陈秀春,王玉,史卫峰,于爱莲   

  1. 泰山医学院病原生物学教研室(山东 泰安 271000)
  • 收稿日期:2009-09-09 出版日期:2010-03-01 发布日期:2010-03-01
  • 通讯作者: 于爱莲,Email: zh.tsmc@yahoo.com.cn
  • 作者简介:张忠(1976-),男,博士,副教授,主要从事媒介生物学研究。
  • 基金资助:

    国家自然科学基金(30671825); 泰山医学院博士科研启动基金

Impact evaluation of frozen storage of Lucilia sericata pupae on the development and prolificacy of descendants of Nasonia vitripennis

 ZHANG Zhong, SONG Guang-Le, YU Guang-Fu, ZHUANG Dong-Ming, WAN Wen-Ju, ZHAO Yang-Hui, CHEN Xiu-Chun, WANG Yu, SHI Wei-Feng, YU Ai-Lian   

  1. Department of Etiology, Taishan Medical University, Taian 271000, Shandong Province, China
  • Received:2009-09-09 Online:2010-03-01 Published:2010-03-01
  • Contact: YU Ai-lian, Email: zh.tsmc@yahoo.com.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China (No. 30671825) and Doctoral Research Start?up Funding of Taishan Medical College 

摘要:

目的 探讨冷冻丝光绿蝇蛹对丽蝇蛹集金小蜂繁殖的影响。方法 考查丽蝇蛹集金小蜂在冷冻丝光绿蝇蛹内的发育历期、后代大小和雌蜂寿命、后代的寄生和繁殖能力。结果 丽蝇蛹集金小蜂在-20 ℃和 -70 ℃冷冻后不同时间丝光绿蝇蛹内的发育历期约为13 d,后代雌蜂体长约为1.8 mm,雄蜂体长约为1.2 mm,后代雌蜂寿命约为11 d,与对照组比较差异无统计学意义(P>0.05);冷冻丝光绿蝇蛹中羽化出的丽蝇蛹集金小蜂对正常丝光绿蝇蛹的寄生率为83%~90%,寄生后蝇蛹的出蜂率为73%~80%,每蛹出蜂数约为37只,雌蜂比90%左右,与对照组比较差异也无统计学意义(P>0.05)。结论 冷冻丝光绿蝇蛹对丽蝇蛹集金小蜂后代的发育和繁殖能力均无显著影响。

关键词: 冷冻, 丝光绿蝇蛹, 丽蝇蛹集金小蜂, 发育, 繁殖

Abstract:

Objective The study was conducted to determine the effects of frozen storage of Lucilia sericata pupae on the prolificacy of Nasonia vitripennis. Methods The length of growth stages, sizes of descendants, life span of the females, parasitic and  reproductive  ability  of  descendants  of  N. vitripennis  within  L. sericata  pupae  were  documented. Results Two  groups of N. vitripennis in L. sericata pupae, frozen at -20 ℃ and -70 ℃, respectively, demonstrated a growth period of approximately 13 days, the body length of female descendants about 1.8 mm and males about 1.2 mm, the life span of female descendants about 11 days, suggesting  no  significant  differences  when  compared  to  the  control  group (P>0.05).  A  total  of  83% to 90% eclosed N. vitripennis could successfully parasitize natural L. sericata pupae, and 73% to 80% emerged, about 37 from each pupa, with the female to male ratio of about 90%, suggesting no significant differences when compared to the control group (P>0.05). Conclusion Frozen storage of L. sericata pupae had no effects on the development and prolificacy of N. vitripennis descendants.

Key words: Frozen storage, Lucilia sericata pupae, Nasonia vitripennis, Development, Prolificacy

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