关键词: 淡色库蚊, 非特异性酯酶, 抗药性

Abstract:

Objective To explore the effect of frozen storage on non-specific esterase(NSE)activity of Culex pipiens pallens and provide a basis for the generalization and application of insecticide-resistance detection of mosquito by biochemical method.Methods The NSE activity of single mosquito was determined based onβ-naphthalene acetic acid ester(β-naphthyl acetate,β-NA)as substrate and solid blue B salt(fast blue B salt)as developer.Results The NSE activity of DDVP-resistant strain was obviously higher than that of susceptible strain.For the A value of NSE activity from Cx.pipiens pallens after frozen for 0,7,14,21 and 28 days at-20℃,the F value of 4 instar larvae of susceptible strain mosquito was 0.7642 by the complete randomized design variance comparison and the F value of 3-day-old adult female of susceptible strain mosquito was 0.7452.The F value of 4 instar larvae of DDVP resistant strain mosquito was 0.1092,and the F value of 3-day-old adult female of DDVP-resistant strain mosquito wa...更多s 0.1521.All of the P value were greater than 0.05 and no significant difference was found.Conclusion There was no difference between on the NSE activity of 4 instar larvae and 3-day-old adult female of Cx.pipiens pallens after frozen for 28 days,and mosquitoes collected from the field could be taken to the lab for batch determination.

Key words: Culex pipiens pallens, Non-specific esterase, Resistance