中国媒介生物学及控制杂志 ›› 2009, Vol. 20 ›› Issue (6): 558-560.

• 论著 • 上一篇    下一篇

ICEstimator在蚊虫毒理学半数致死浓度计算上的应用

李雨春1,杜尊伟2,王学忠2,张再兴2   

  1. 1 海南省疾病预防控制中心寄生虫病防治所(海口 570203); 2 云南省寄生虫病防治所
  • 收稿日期:2009-08-19 出版日期:2009-12-20 发布日期:2009-12-20
  • 通讯作者: 张再兴,Email: zzxsm@126.com
  • 作者简介:李雨春(1983-),男,硕士, 从事寄生虫及蚊虫毒理学研究。

Application of ICEstimator in the mosquito toxicology to calculate median lethal concentration

 LI Yu-Chun, DU Zun-Wei, WANG Xue-Zhong, ZHANG Zai-Xing   

  1. Hainan Center for Disease Control and Prevention, Haikou 570203, Hainan Province, China
  • Received:2009-08-19 Online:2009-12-20 Published:2009-12-20
  • Contact: ZHANG Zai-xing, Email: zzxsm@126.com

摘要:

【摘要】 目的 探索ICEstimator用来计算半数致死浓度(LC50)值及其95%可信区间(95%CI)的可行性和优越性。方法利用ICEstimator分别计算敌百虫对白纹伊蚊、致倦库蚊的LC50及其95%CI和氟氯氰菊酯对白纹伊蚊、致倦库蚊的LC50及其95%CI,并与目前常用的计算方法(SPSS、SAS、DPS)对上述数值进行分析比较。结果 经DPS/SAS/SPSS软件计算氟氯氰菊酯对白纹伊蚊及致倦库蚊的LC50(mg/ml)及其 95%CI分别为5.03(4.68~5.38)、5.20(4.90~5.60)、5.14(4.83~5.46)、5.14 (4.83~5.46)和5.31(4.58~6.03)、5.40(4.75~6.15)、5.28(2.37~7.11)、5.28(2.37~7.11);敌百虫对白纹伊蚊及致倦库蚊的LC50(mg/ml)及其 95%CI分别为92.92 (83.27~102.58)、100.60(90.60~110.70)、96.00(87.88~105.33)、96.00(87.88~105.33)和1123.02(998.89~1247.14)、1123.70(800.60~1652.40)、1111.91(725.47~1745.88)、1111.90(725.46~1745.87)。经非参数K?W?Test检验,二者计算的数值及其95%CI差异均无统计学意义(χ2=5.595, P=0.113)。结论 用ICEstimator可快速得到某杀虫剂对某种蚊虫的LC50及其95%CI,与SPSS和SAS方法比较,该计算方法计算过程简单,速度快,更加有效地利用了初始数据。

关键词: 半数致死浓度值, ICEstimator

Abstract:

【Abstract】 Objective  To explore feasibility and superiority of ICEstimator online software in the calculation of median lethal concentration(LC50) and its 95% confidence interval(95%CI). Methods The LC50 values of dipterex and beta?cyfluthrin to Aedes albopictus and Culex pipiens quinquefasciatus were calculated by ICEstimator, SPSS 13.0, SAS 9.0 and DPS 7.55, respectively. Results The  LC50  value  of  beta?cyfluthrin  to  Ae. albopictus  and  Cx. pipiens quinquefasciatus  and  its  95%CI, calculated by the above mentioned softwares , were 5.03(4.68-5.38), 5.20(4.90-5.60), 5.14(4.83-5.46), 5.14 (4.83-5.46), 5.31(4.58-6.03), 5.40(4.75-6.15), 5.28 (2.37-7.11), 5.28(2.37-7.11), and for dipterex, that were 92.92(83.27-102.58), 100.60(90.60-110.70), 96.00(87.88-105.33), 96.00(87.88-105.33) and 1123.02(998.89-1247.14), 1123.70(800.60-1652.40), 1111.91(725.47-1745.88), 1111.90(725.46-1745.87), respectively.  However,  there  were  no  significant  difference  among three  groups  of   median  lethal  concentration  value  (χ2=5.595, P=0.113)  and  their  95%CI by K?W?Test. Conclusion The LC50 value and its 95%CI could be got fast and conveniently by ICEstimator compared to other methods.

Key words: Median lethal concentration(LC50) value, ICEstimator 

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