关键词: 法医昆虫学, 死后间隔时间, 丝光绿蝇, 头咽骨, 气门

Abstract:

　　【Abstract】　Objective　Survey the morphological changes of Lucilia sericata larvae at different temperature and screen out the indexes deducing larvae age. Methods　The necrophagous fly L. sericata were reared at constant temperature of 20 ℃，24 ℃ and 28 ℃ respectively and were tempted to lay eggs with lean pork. One Sample was taken at 12 h intervals till 50% of the larvae were in pupae stage. Then, the anterior spiracle，posterior spiracle and cephalopharyngeal skeleton were investigated through digital image processing combined with morphologic observation. Results　The anterior spiracle，posterior spiracle and cephalopharyngeal skeleton regularly changed with daily larval growth. The three indexes including the sclerotizes area of pharyngeal sclerite, the average optical density of pharyngeal sclerite and the posterior spiracle could be used as the ideal indexes of larvae age. Conclusion　The regular changes of the three sections at constant temperature would provide some scientific database for the exact deducing of the postmortem interval.

Key words: Forensic entomology, Postmortem interval, Lucilia sericata, Cephalopharyngeal skeleton, Spiracle