中国媒介生物学及控制杂志 ›› 2009, Vol. 20 ›› Issue (6): 528-530.

• 论著 • 上一篇    下一篇

溴氰菊酯和辛硫磷亚致死剂量对德国小蠊酶活性的影响

竹傲,游红,朱福兴   

  1. 华中农业大学植物科学技术学院(湖北 武汉  430070)
  • 收稿日期:2009-06-21 出版日期:2009-12-20 发布日期:2009-12-20
  • 通讯作者: 朱福兴, Email: zhufuxing@mail.hzau.edu.cn
  • 作者简介:竹傲(1982-),男,硕士,从事农药药理学研究。

Effect of sublethal doses of deltamethrin and phoxim on enzyme activity of Blattella germanica

 ZHU Ao, YOU Hong, ZHU Fu-Xing   

  1. Huazhong Agricultural University, Wuhan 430070, Hubei Province, China
  • Received:2009-06-21 Online:2009-12-20 Published:2009-12-20
  • Contact: ZHU Fu-xing, Email: zhufuxing@mail.hzau.edu.cn

摘要:

【摘要】 目的 研究溴氰菊酯和辛硫磷亚致死剂量处理后德国小蠊羧酸酯酶、谷胱甘肽S-转移酶、乙酰胆碱酯酶的活性变化规律。方法 采用分光光度计离体测定酶的活性,应用DPS软件进行统计分析。结果 辛硫磷亚致死剂量处理德国小蠊后24 h,羧酸酯酶活性降到最低,与处理前相比降低了79.33%,24 h后其活性逐渐恢复,168 h达到处理前的37.10%;溴氰菊酯亚致死剂量处理后,羧酸酯酶活性略有下降;经溴氰菊酯和辛硫磷亚致死剂量处理后,谷胱甘肽S-转移酶的活性略有下降,72 h后其活性分别为处理前的74.97%和79.77%;经溴氰菊酯亚致死剂量处理后,乙酰胆碱酯酶活性24 h略有升高,随后逐渐下降,经辛硫磷处理后其活性略有降低。结论 经溴氰菊酯和辛硫磷亚致死剂量处理后德国小蠊体内酶活性在不同的时间与处理前存在差异。

关键词: 德国小蠊, 亚致死剂量, 羧酸酯酶, 谷胱甘肽S-转移酶, 乙酰胆碱酯酶

Abstract:

  【Abstract】 Objective Activities of carboxylesterase,glutathione S-transferase and acetylcholinesterase from German cockroach (Blattella germanica) treated with sublethal doses of deltamethrin and phoxim were measured. Methods Enzyme activities were measured in vitro by spectrophotometry and DPS was used for statistical analysis. Results  Activity of carboxylesterase reduced to the lowest level at 24 h after treated with sublethal doses of phoxim. The activity decreased 79.33% compared with that before treatment, then recovered gradually and at 168 h reached 37.10% of that of before treatment. Activity of carboxylesterase reduced slightly after treated with sublethal doses of deltamethrin. For glutathione S-transferase, activities declined slightly and were 74.97% and 79.77% after treatment for 72 h , respectively. Activity of acetylcholinesterase increased within 24 h after treated with sublethal doses of deltamethrin, then declined gradually. Activity reduced slightly after treated with sublethal doses of phoxim. Conclusion Compared with before treatment, the enzyme activity of German cockroach changes with the difference of time after treated with sub-lethal doses of deltamethrin and phoxim.

Key words: Blattella germanica, Sub-lethal dosage, Carboxylesterase, Glutathione S-transferase, Acetylcholinesterase

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