中国媒介生物学及控制杂志 ›› 2009, Vol. 20 ›› Issue (3): 187-188.

• 论著 • 上一篇    下一篇

冰冻储存对淡色库蚊非特异性酯酶活性的影响

李士根1,王霄2,谭文彬1,甄天民3,全芯1,高红刚1,刘永春1   

  1. 1 济宁医学院寄生虫学教研室(山东 济宁 272013); 2 济宁医学院外总教研室; 3 山东省医学科学院
  • 收稿日期:2008-12-19 出版日期:2009-06-20 发布日期:2009-06-20
  • 作者简介:李士根(1966-),男,山东人,博士,副教授,主要从事医学寄生虫学教学与研究。
  • 基金资助:

    山东省卫生厅资助项目(2001CA2CEB1)

Effect of frozen storage on non?specific esterase activity of Culex pipiens pallens

LI Shi-Gen, WANG Xiao, TAN Wen-Bin, ZHEN Tian-Min, QUAN Xin, GAO Hong-Gang, LIU Yong-Chun   

  1. Parasitology Department of Jining Medical College, Jining, Shandong 272013, China
  • Received:2008-12-19 Online:2009-06-20 Published:2009-06-20

摘要:

  【摘要】 目的 探讨冰冻储存对淡色库蚊非特异性酯酶(non-specific esterase, NSE)活性的影响,为蚊虫抗药性生物化学检测方法的推广应用提供依据。方法 以β-乙酸萘酯为底物,坚固蓝B盐为显色剂,测定单个蚊虫NSE活性。结果DDVP抗性品系淡色库蚊NSE活性水平明显高于敏感品系;经完全随机设计的方差分析比较,-20 ℃冰冻0、7、14、21、28 d的淡色库蚊NSE活性A值之间的差别,敏感品系4龄幼虫的F值为0.7642,该品系3日龄雌成虫的F值为0.7452,DDVP抗性品系4龄幼虫的F值为0.1092,该品系3日龄雌成虫的F值为0.1521,P值均>0.05,差异无统计学意义。结论 -20 ℃冰冻28 d对淡色库蚊4龄幼虫和3日龄雌成虫体内NSE活性无影响,可在现场采集蚊虫带回实验室集中测定。

关键词: 淡色库蚊, 非特异性酯酶, 抗药性

Abstract:

  【Abstract】 Objective To explore the effect of frozen storage on non-specific esterase (NSE) activity of Culex pipiens pallens and provide a basis for the generalization and application of insecticide-resistance detection of mosquito by biochemical method. Methods The NSE activity of single mosquito was determined based on β-naphthalene acetic acid ester (β-naphthyl acetate, β-NA) as substrate and solid blue B salt (fast blue B salt) as developer. Results The NSE activity of DDVP-resistant strain was obviously higher than that of susceptible strain. For the A value of NSE activity from Cx.pipiens pallens after frozen for 0, 7, 14, 21 and 28 days at -20 ℃, the F value of 4 instar larvae of susceptible strain mosquito was 0.7642 by the complete randomized design variance comparison and the F value of 3-day-old adult female of susceptible strain mosquito was 0.7452. The F value of 4 instar larvae of DDVP resistant strain mosquito was 0.1092, and the F value of 3-day-old adult female of DDVP-resistant strain mosquito was 0.1521. All of the P value were greater than 0.05 and no significant difference was found. Conclusion There was no difference between on the NSE activity of 4 instar larvae and 3-day-old adult female of Cx.pipiens pallens after frozen for 28 days, and mosquitoes collected from the field could be taken to the lab for batch determination.

Key words: Culex pipiens pallens, Non-specific esterase, Resistance

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