中国媒介生物学及控制杂志 ›› 2009, Vol. 20 ›› Issue (1): 15-17.

• 论著 • 上一篇    下一篇

白纹伊蚊唾液腺细胞的原代培养

施海瑛张玲敏李薇孙家梅   

  1. 广东省暨南大学医学院寄生虫教研室(广州 510632)
  • 收稿日期:2008-08-29 出版日期:2009-02-20 发布日期:2009-02-20
  • 通讯作者: 张玲敏,Email:tzhanglm@jnu.edu.cn
  • 作者简介:施海瑛(1977-),女,江西省信丰人,硕士研究生,主要从事白纹伊蚊的研究。

The  primary  culture  of  salivary  gland  cells  from  Aedes albopictus  in  vitro

 SHI Hai-Ying, ZHANG Ling-Min, LI Wei, SUN Jia-Mei   

  1. The  primary  culture  of  salivary  gland  cells  from  Aedes albopictus  in  vitro
  • Received:2008-08-29 Online:2009-02-20 Published:2009-02-20
  • Contact: ZHANG Ling-min, Email:tzhanglm@jnu.edu.cn

摘要:

  【摘要】 目的 探讨白纹伊蚊唾液腺细胞的培养,试图建立白纹伊蚊唾液腺细胞的原代和传代培养。方法 采用Schneider 培养基添加10%的胎牛血清和双抗﹙100 U/ml青霉素,100 μg/ml链霉素﹚作培养液,在无菌条件下取出唾液腺,经PBS液清洗后放入培养液中,置26 ℃培养箱中培养。结果   3 d后白纹伊蚊唾液腺组织开始长出单个细胞,6 d后单个细胞增多。说明白纹伊蚊唾液腺细胞在体外培养条件下能够存活一段时间。结论 白纹伊蚊唾液腺细胞体外培养存活。

关键词: 白纹伊, 唾液腺, 原代培养

Abstract:

  【Abstract】 Objective To investigate the culture of salivary gland cells from adult Aedes albopictus, and try to establish its primary culture and passage culture. Methods Salivary glands dissected under sterile condition were kept in Schneider medium containing 10% fetal bovine serum, 100 U/ml penicillin and 100 μg/ml phytomycin, and cultured at 26 ℃ in the incubator. Results After incubation for three days, single cells could be observed around salivary gland, and the quantity of single cell increased after six days. It showed that the culture of salivary gland cells from Ae.albopictus adult could survive for a period in vitro. Conclusion The culture of salivary gland cells from Ae.albopictus adult could survive in vitro.

Key words: Aedes albopictus, Salivary gland, Primary culture

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