关键词: CoⅠ基因, DNA条形码, DNA芯片, 蚊虫

Abstract: Objective To validate the feasibility of the high effective quick method establishment for the medical vector mosquito identification by DNA chip. Methods Thirty COⅠ gene partial sequences of fifteen mosquitoes which belong to five genus were analyzed. The homologous gene sequences of fifteen sequences were searched by motif and a DNA barcode chip in silico was generated. The thirty COⅠ gene partial sequences were hybridized in silico, and the identities of different genus, different species in the same genus and different samples of one specie were analyzed by Bio-Edit. The results of hybridized in silico and DNA sequence were clustered and compared using maximum parsimony method by phylip 3.66. Results A DNA barcode chip in silico with seventy-eight DNA fragment was generated. The average identity of different genus was 0.75, and the average identity of different species in the same genus was 0.84, and the average identity of different samples in the same specie was 0.98. The cluster from the results of hybridized in silico was consistent with the cluster from DNA sequence. Conclusion The DNA barcode chip can identify the fifteen mosquitoes in silico, and integrating the DNA barcode and DNA chip is feasible.