关键词: 鼠疫耶尔森菌, IS285, 聚类

Abstract: Objective To know about the genetic characteristics of a new kind of Yersinia pestis, isolated at Yulong, Yunnan province of China, and to analyze the relationship between these strains and the other types of Y.pestis of China. Methods The primers were designed according to six IS285 sites of DNA genome of CO92 strain. The differences between Yulong strains and other strains of Y.pestis were analyzed by PCR and cluster analysis. Results Compared to CO92 strain, Chinese strains had some variation at some IS285 sites. 4 of the 5 Yulong strains had the same profile of IS285, while the other one presented variation at one site. On the bases of PCR, the types of ChineseY.pestis could be clustered as 8 groups. The 5 Yulong strains were divided into two groups. Four strains of them, plus Dianxi zonggu and Dianmin jumin, most of strains isolating from Marmota baibacina, and all of strains isolating from Marmota himalayana belongs to one group. Conclusion The genetic characteristic of Y.pestis from Yulong was known primarily, suggesting the importance of Yulong strains during the prevalence and evolution of Chinese Y.pestis strains.