关键词: 家蝇幼虫, 血细胞, 形态学, 吖啶橙, 碘化丙啶

Abstract: Objective The hemocytes of the 3^rd instar larva of Musca domestica were observed and classified with fluorescent staining methods and phase contrast microscope. Methods The hemocytes of the 3^rd instar larva of M.domestica were stained by acridine orange(AO) and propidium iodide(PI) and the morphologic characteristics were observed and classified. Results (1) The hemocytes of the 3^rd instar larvae of M.domestica were divided into prohemocyte, plasmatocyte, granulocyte, sphrulocyte and oenocytoid. The plasmatocytes were divided into big nucleus hematocyte and micronucleus hematocyte. (2) The prohemocyte was easily identified with phase contrast microscope, but its character was not notable by fluorescent staining methods. (3) The granulocyte and big nucleus plasmatocyte, sphrulocyte and micronucleus plasmatocyte were confusable with phase contrast microscope, but they were easily distinguished with fluorescent microscope. Conclusion The fluorescent staining methods combined with phase contrast microscope can be used to observe unknown morphologic characterstics of the insect hemocytes, and the hemocytes of the 3^rd instar larva of M.domestica could be accurately divided into five types.