关键词: 美洲大蠊, 消化酶, 活性

Abstract: Objective To compare the digestive enzyme activity between laboratory American cockroach and feral American cockroach.Methods American cockroaches were anatomized to obtain midgut of the 2-3 instar and 9-10 instar nymphs and adults,the activities of proteases,amylases,lipases and sucrases were tested.Results The activities of protease of laboratory reared American cockroach were 56.28,59.75,65.22 U in 2-3 instar and 9-10 instar nymphs and adults,the activities of lipase were 47.67,69.91,79.44 U;the activities of amylase were 51.16,76.40,95.51 U,the activities of sucrase were 0.60,0.67,0.97 U;the activities of protease of feral American cockroach were 31.68,42.49,44.03 U in 2-3 instar and 9-10 instar nymphs and adults,the activities of lipase were 56.37,76.38,87.16 U,the activities of amylase were 47.15,57.90,72.44 U,the activities of sucrase were 0.91,0.96,1.22 U.Conclusion The activities of protease and amylase of cultured American cockroach were higher than feral American cockroach of the same growth phase;but the activities of lipase and sucrase were lower.During the development of American cockroach,the activities of protease,lipase,amylase and sucrase were stronger gradually.