关键词: 鼠疫菌, 多重聚合酶链反应, F1抗原, pla基因, inv基因

Abstract: Objective To apply a rapid and special method for the detection of Yersinia pestis by multiplex polymerase chain reaction(PCR).Methods The F1 antigen,pla gene,inv gene and a specific segment on the chromosome were co-amplified by PCR.An internal control(IC)was required in order to prevent false negative results that might be caused by PCR inhibitors.Other enteric pathogenic bacteria DNA were amplified as control.Results The amplicons of Yersinia pestis DNA were the same with the anticipative products.Only internal control amplicon was gotten while amplifying other enteric pathogenic bacteria DNA.Conclusion This method is specific and rapid and can identify Yersinia pestis.It is useful for detection of Yersinia pestis.