中国媒介生物学及控制杂志

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淡色库蚊对残杀威抗性与酯酶和乙酰胆碱酯酶的关系研究

李春晓;董言德;赵彤言   

  1. 军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室 北京100071
  • 出版日期:2007-02-20 发布日期:2007-02-20

Relationship of esterase,acetylcholinesterase and propoxur-resistance to Culex pipiens pallens

LI Chun-xiao; DONG Yan-de; ZHAO Tong-yan   

  1. State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology Academy of Military Medical Sciences, Beijing 100071, China
  • Online:2007-02-20 Published:2007-02-20

摘要: 目的 研究淡色库蚊对残杀威抗性与酯酶和乙酰胆碱酯酶的关系。方法 WHO浸渍法检测蚊虫抗性指数;分光光度法检测羧酸酯酶和乙酰胆碱酯酶活性。结果 使用残杀威对淡色库蚊野外品系进行筛选,筛选8代后抗性指数由原来的4.77倍上升至12.07倍。对敏感品系和抗性品系分别进行羧酸酯酶和乙酰胆碱酯酶活性测定,二者的羧酸酯酶活性差异无统计学意义(P>0.05),而二者的乙酰胆碱酯酶活性在高浓度残杀威抑制浓度(100×10-6、10×10-6、1×10-6)下,抑制率敏感品系分别为(93.92±0.65)%、(91.69±0.54)%和(87.03±1.14)%,抗性品系分别为(89.81±0.92)%、(87.41±1.66)%和(83.84±0.36)%,差异具有统计学意义(P<0.05)。结论 羧酸酯酶在对残杀威的抗性形成中作用较小,而乙酰胆碱酯酶的敏感性降低与抗性品系的抗性形成有关。

关键词: 淡色库蚊, 残杀威, 羧酸酯酶, 乙酰胆碱酯酶

Abstract: Objective To study the activity of relationship between esterase,acetylcholinesterase and the resistance to propoxur in the larva of Culex pipiens pallens. Methods WHO bioassay and spectrophotometric method were used. Results A strain from field selected using propoxur in lab for generations with 12.07-fold resistance ratio was abtained,which the parent was 4.77-fold. The difference of esterase activity between sensitive and resistant strain was not significant. Otherwise,the acetylcholinesterase activity between two strains existed significant difference at high concentration (100×10-6,10×10-6 and 1×10-6) propoxur. The inhibiting rate was (93.92±0.65)%,(91.69±0.54)% and (87.03±1.14)% respectively in sensitive strain,and (89.81±0.92)%,(87.41±1.66)% and (83.84±0.36)% respectively in resistant strain. Conclusion Metabolic detoxification induced by esterase play a minor role in propoxur-resistant strain. The insensitive AChE may be the main factor in related to the propoxur resistance.