关键词: 流行性乙型脑炎病毒, 蚊媒, 检测

Abstract: Objective To investigate species composition,distribution and the situation of mosquito vectors of Japanese encephalitis virus(JEV) in Zhuhai area,hereby to provide scientifical evidences for strengthening monitoring,preventing and controlling the Japanese encephalitis. Methods Mosquitoes were trapped at 4 hoggeries and ports in Zhuhai from January,2005 to June,2006,and provided by Zhuhai CDC. The samples of mosquito vectors were ground to make suspension,the RNA of virus were extracted directly,or after suspension was inoculated into cells of C6/36s and educated,and then RT-PCR was carried out; The positive products were cloned and sequenced. Results 876 adult mosquitoes were captured during 2005 in the registered hoggeries. They belonged to 4 genera and 8 species,of which Culicinae species were the most dominant species.Cx.tritaeniorhynchus accounted for 35.73 ,Cx.quinquefasciatus 32.54 andCx.sitiens 21.35.542 adult mosquitoes were captured in the ports of Zhuhai. Among them,82.47 were Aedes albopictus,16.25 were Cx.tritaeniorhynchus. 1 positive sample was identified as a mild JEV by fluorometric PCR from the mosquitoes(Cx.sitiens) in the registered hoggeries and the ports of local area. It is accordant to the fact of low local epidemic JE in Zhuhai. Conclusion Despite of the low local epidemic of JE in Zhuhai,the main vectors of JE exist and are dominant in Zhuhai,the prevention and control of JE can't be overlooked.