关键词: 提取方法, 伤害, 细胞

Abstract: Objective To compare the two mothods of isolating the antimicobial peptides from Musca domestica larvae.Mothods Musca domestica larvae were infected with Escherichia coli by a sting,then the antimicrobial peptides were extracted from the insect.After that,the antimicrobial peptides were isolated by grinding,sephadex(G-50) gel filtration.The last,the anticancer activity of the peptides worked upon K562 cell and lymph cell were detected by Flow cytometer(FCM).Results The two kinds of peptides from Musca domestica isolated by the different methods could kill K562 cell to some extent,but they are different in working upon normal cell.Physiological saline influence cell less than the buffer.Conclusion(Buffer could) make a high osmotic pressure and impel cell to death;physiological saline(don't) do as the buffer.