关键词: 家蝇, 过氧化氢酶, 抗药性, 敌敌畏, 高效氯氰菊酯

Abstract: Objective To test the effect of dichlorvos and beta-cypermethrin on catalase activity,and analyse their resistance evolution with different insecticide application methods,and to provide evidence for Musca domestica control.Methods Apply insecticides to M.domestica at adulthood or larvae phase,LD₅₀ of different generation of M.domestica were tested,and the index of fastness was calculated,and test the CAT activities of M.domestica on different generations,analyse trends of change.Results More change on activities of CAT happened on groups which touched insecticides at larvae phase and groups which were exposed dichlorvos.The high tops((DR2-6)、(LR2-8)) reach(37.157 5 U/mg) and(28.140 7 U/mg),the difference have meanings on statistics((P<0.002 5),P<0.005);the activities of CAT of groups which touch dichlorvos changes more,especially touch insecticides at larvae phase,the highest((DR2-6,37.157 5 U/mg)) and lowest point((DR2-8),(6.566 2 U/mg)) of the test appeared in this group,the three point((DR2-8,6.566 2 U/mg);(DR1-8,11.031 0 U/mg);(DR2-10,12.446 1 U/mg)) which lower than before all appeared in the group which touch dichlorvos.Conclusion There are more effects on M.domestica when it met insecticides on larvae phase;compared with alpha-cypermethrin,we presume connections between activities of CAT and Dichlorvos are more.