中国媒介生物学及控制杂志

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家蝇幼虫抗菌蛋白的抗菌特性及其组成

万启惠;刘晖;贺莉芳;张曦   

  1. 遵义医学院寄生虫学教研室 遵义563003
  • 出版日期:2006-06-20 发布日期:2006-06-20

Antibacterial Characterization and Component of the Antibacterial Proteins in Musca domestica Larva by Different Inducements

WAN Qi-hui;LIU Hui; HE Li-fang; ZHANG Xi   

  1. Department of Parasitology,Zunyi Medical College, Zunyi 563003,China
  • Online:2006-06-20 Published:2006-06-20

摘要: 目的 观察不同诱导源诱导家蝇幼虫产生抗菌蛋白的抗菌特性及其蛋白组分。方法 取5种诱导源诱导后提取抗菌蛋白,用不同细菌进行抑菌实验,观察5种诱导源诱导组抗菌蛋白的抗菌特性;5种诱导源诱导组抗菌蛋白经SDS聚丙烯酰胺凝胶电泳,观察抗菌蛋白的组分变化。结果 各诱导组产生的抗菌蛋白均能抗多种G+菌、G-菌和耐药菌株,但对不同细菌的抗菌活性有所差别。各诱导组抗菌蛋白中有2条电泳带与对照组完全相同,但含量高于对照组;各诱导组与对照组比较均出现不同的新的蛋白带。结论 家蝇幼虫诱导后产生的抗菌蛋白抗菌谱广;不同诱导源可诱导家蝇幼虫产生相同蛋白及不同的新蛋白。

关键词: 家蝇幼虫, 抗菌蛋白, 诱导源, 抗菌活性, 电泳分析

Abstract: Objective To observe the antibacterial characterization and component of the antibacterial proteins in the Musca domestica larva by different inducements.Methods Several different bacteria were used as detecting index to detect the antibacterial activity of antibacterial proteins which is induced by five inducements.SDS-PAGE was used as detecting index to observe the proteins compared with the control group.Results These antibacterial proteins could inhabit many bacteria(including some Grame-positive,Grame-negative bacteria and rifampin resistant E.coli),but the antibacterial activities were different.The antibacterial proteins induced by five inducements had two same component compared with the control group,but their content were different,and different new proteins appeared at differernt induced group.Conclusion The antibacterial proteins of the Musca domestica larva by different inducements could inhibit diversiform bacteria.Different inducements could induce same proteins and different new proteins.