中国媒介生物学及控制杂志

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油茶皂素不育剂对鼠类抗生育功能的实验研究

陈剑锋;何晓玲;李锋;陈浩;吴锦忠;郭养浩   

  1. 福州大学药物生物技术与工程研究所 福州350002
  • 出版日期:2006-02-20 发布日期:2006-02-20

Experimental Research on Antifertility Function of Sasanqua Saponin Sterilant Against Rodent

CHEN Jian-feng; HE Xiao-ling; LI Feng; CHEN Hao; WU Jin-zhong; GUO Yang-hao   

  1. Institute of Pharmaceutical Biotechnology and Engineering,Fuzhou University,Fujian Province,Fuzhou 350002, China
  • Online:2006-02-20 Published:2006-02-20

摘要: 目的 研究油茶皂素不育剂对鼠类的抗生育功能。方法 测定不同浓度油茶皂素不育剂对雄性小鼠的体外杀精效果和体内抗生育能力。结果 油茶皂素不育剂对雄性小鼠呈现出较优的剂量依赖性体外杀精活性,3 min时的最低杀精浓度为0.05 mg/ml,与人用杀精剂壬苯醇醚相当,比扁桃酸(0.1 mg/ml)和柠檬酸(0.25 mg/ml)低。与生理盐水对照组比较,125 mg/(kg·d)油茶皂素不育剂灌胃给药4周后,可以明显降低雄性小鼠的活精子数(92.1%)和精子活力(52.6%),可以显著减少雌性小鼠的妊娠率(64.0%)和活胎数(67.4%)。结论 油茶皂素不育剂具有较强的体外杀精活性和体内抗生育功能,可进一步开发成体外杀精避孕药和环境友好型鼠类抗生育药剂。

关键词: 油茶皂素, 不育剂, 鼠类, 杀精作用, 抗生育功能

Abstract: Objective To study the antifertility function of sasanqua saponin sterilant against rodents.Methods The spermicidal effect in vitro and the antifertility ability in vivo on male mice of the sasanqua saponin sterilant in different concentration were mensurated.Results The spermicidal effect in vitro on male mice of the sasanqua saponin sterilant was provided with excellent positive relation in dosage-effect.The minimum spermicidal concentration being(0.05 mg/ml) in(3 min) of the sasanqua saponin sterilant was corresponded with nonoxynol spermicide and was lower than mandelic acid((0.1 mg/ml)) and citric acid((0.25 mg/ml)).Compared with the control group,after ig(125 mg/(kg·d)) sasanqua saponin sterilant for 4 weeks,(92.1%) alive sperm and(52.6%) sperm motility on male mice could be obviously reduced,and(64.0%) gravidity rate and(67.4%) alive embryo on female mice could be significantly decreased.Conclusion The sasanqua saponin sterilant was provided with obvious spermicidal effect in vitro and antifertility function in vivo.The sasanqua saponin spermicide or prophylactic and the antifertility medicament against rodents with entironment amity should be exploited in future.