关键词: 褐家鼠, 遗传多态性, 随机扩增多态DNA, 汉坦病毒

Abstract: Objective To understand the association between the genetic variation of hantavirus and genetic differentiation of Rattus norvegicus population from Beijing.Methods The partial M segment of hantaviruses from captured rodent lung samples were amplified and then sequenced.The alignment of the nucleotide and phylogenetic analysis were made with DNASTAR software.RAPD-PCR was used to analyze the DNA polymorphism of R.norvegicus populations.Results(The 356) bp M segment obtained from 11 representative samples were all identified as SEOV,with some degree of heterogeneity(0.1%8.0%) between the different geographical locations.Phylogenetic analysis revealed that there exists at least 2 genetical distinct hantaviruses lineages.BjFT01 strain virus represented a mono-phylogentic lineage.62 clear polymorphic bands received with 4 out of 50 random primers presented the polymorphism.The cluster of analysis showed that the samples can be divided in to different groups.The individual(XFD population) from Fengtai district 2 had a special band and formed a distinct cluster.The inheritic distance of XFD population was larger than between others and was relatively independent group.Comparison of phylogenies inferred from HV and hosts showed the match phylogenies of BjFT01 strain and XFD population.Conclusion(RAPD marker) reveals the population differentiation of R.norvegicus and has some to extend association with HV variation and distribution at in Beijing.These data suggest that BjFT01 HV and their rodents hosts population share the common differentiation history.It's farther approved that Beijing Seoul virus is more probability to be transmitted from other place with the rat hosts.