关键词: 巴尔通体, 聚合酶链反应, 蚤

Abstract: Objective Most known Bartonella.species are arthropod borne and cat fleas can carry and transmit this organism from cats to cats. The study intended to make clear whether someBartonellaspecies,which are emerging pathogens,could be carried or transmitted by fleas in natural enviroments in China. Methods In this study,fleas were collected from domestic cats,dogs and rats hosts from June to July 2003 in Yunlong county,Yunnan province. PCR assay with genus. Bartonella specific primers were applied to Bartonelladetection in every group of fleas which were same species and came from one captured animal. Results Ctenocephalides felis,C.orientis,Pulex irritans,Xenopsylla cheopis,Leptopsylla segnis,Ctenophthalmus quadratus,Neopsylla specialis specialiscollected from the host animals were 251.One group of Ct.felis including 8 tested fleas were PCR positive with three pairs of primers for Bartonella species.FiveLeptopsylla segnisfrom a Rattus tanezumi flavipectus were also amplified DNA fragments of Bartonella species. Conclusion These findings indicate that fleas associated with cats and rats can carry Bartonella species and may play an important role in this pathogen transmission among animals and humans in China.