关键词: 鼠疫, 免疫血清, 效价

Abstract: Objective Looking for the methods to produce high titer antiplague serum through immunizing rabbits with weak virulence plague strains,and the affection on the titers of freezing immune serum after different conserved time.Methods(1) One group of rebbits is immunized through multi route including subcutaneous,intramuscular and intravenous injections,another group is immunized only with intravenous route.All rabbits were vaccinated 4 6 times with 1×10¹⁰/ml supension which were made of weak virulence Y.pestis after 48 h culture in 37 ℃.(2) The titers of freezing conserved antiserum was tested with bacterial agglutination method to determine the stability of antibody.Results(1) The antiserum of rabbits immunized with only intravenous route show higher bacterioagglutinotiters than the antiserum of rabbits immunized with multi injection routes.(2) The titers of antiserum are dropping with the conserved time extending.But the titers dropped no more than 2 titers after 1 year.Conclusion High titer immune serum is available from rabbits immunized with weak virulence plague strains through intravenous route.The baterioagglutinotiters of antiplague serum are stable relatively in one year.