肺吸虫模拟抗原诊断价值及序列分析

中国媒介生物学及控制杂志

肺吸虫模拟抗原诊断价值及序列分析

时红波;姜昌富;潘红

华中科技大学同济医学院病原生物学系武汉430030

出版日期:2004-12-20 发布日期:2004-12-20

Study on Diagnostic Value and Sequence of Mimic Antigen of Paragonimus

SHI Hong-bo; JIANG Chang-fu; PAN Hong

Department of Pathogenic Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030,China

Online:2004-12-20 Published:2004-12-20

摘要/Abstract

摘要： 目的探讨噬菌体展示肺吸虫模拟抗原的诊断价值并分析与天然抗原的同源性。方法建立肺吸虫噬菌体模拟抗原的ELISA,对肺吸虫病人、日本血吸虫病人、旋毛虫病人及健康者血清进行检测,分析其敏感性和特异性;提纯噬菌体模拟肽的DNA模板进行序列测定,以Blast软件分析其序列同源性。结果肺吸虫模拟抗原的6个阳性克隆中P5、P6、P8、P13、P16的阳性、阴性预测值及诊断效率均为100%,P7的阳性、阴性预测值及诊断效率分别为100%、78.9%、86.7%。上述6个克隆有2个克隆的DNA序列完全相同,即6个克隆包含了5种不同的抗原表位;Blast分析表明5个表位与已知的肺吸虫抗原表位无DNA序列的同源性。结论肺吸虫模拟抗原对肺吸虫病具有一定的诊断价值,这5种表位可能从空间结构上模拟了天然抗原表位。

关键词: 肺吸虫, 模拟抗原, 表位, DNA序列

Abstract: Objective To study the diagnostic value of mimic antigen of paragonimus and analyze the sequence homology between the mimic antigen and the natural antigen.Methods The phage displayed antigens of paragonimus were used to examine the sera of patients with paragonimiasis,schistosomiasis,trichinosis and healthy people,and their sensitivity and specificity were acquired;The templates of the mimic antigen were purified to determine the sequence and the sequence homology was analyzed by Blast.Results In the six mimic antigens(P5,P6,P7,P8,P13 and P16),the positive expectation,the negative expectation and the diagnostic efficiency of P5,P6,P8,P13 and P16 were all 100%,for P7 was 100%,78.9%,86.7%,respectively.Two of the six clones had the same DNA sequence,so there are five different antigenic epitopes in the six clones;the Blast analysis showed that there were no sequence homology between the five epitopes and the known antigen of paragonimus.Conclusion The mimic antigen of paragonimus had definite diagnostic value in the paragonimiasis,and the five epitopes may mimic the natural antigen in the special structure.

时红波;姜昌富;潘红. 肺吸虫模拟抗原诊断价值及序列分析[J]. 中国媒介生物学及控制杂志.

SHI Hong-bo; JIANG Chang-fu; PAN Hong. Study on Diagnostic Value and Sequence of Mimic Antigen of Paragonimus[J]. Chines Journal of Vector Biology and Control.