关键词: 肾综合征出血热, 双价纯化疫苗, 安全性, 免疫效果

Abstract: Objective In order to estimate the safety and immunological efficacy of new vaccine “bivalent purified vaccine against HFRS(Vero cell)” which was supplied by Bailong Biological Technology Limited Company,Changchun.To provide clue for ensuring Two injection primary immunization procedure and the feasibility of extending.Methods All the inoculated objective were observed by follow up system and local response after each injection,sera were collected before and after inoculation,immunofluorescent antibody assay was used to detect specific IgG antibody,and Mcro CPE method was used to detect the titer of the neutralizing antibody.Results The rates of slight temperature rise and slight local response were 3.66% and 1.83%,respectively,of which side effects were slight and had been faded out in three days.We collected 81 pieces of double serum about before primary immunization and after all the immunization.The IFAT and MCPENT antibody reactions were negative before primary immunization,while twenty one days after the second injection,the rate of seroconversion of the immunofluorescent antibody by IFAT was 95.06%.The rates of seroconversion of type Ⅰ and typeⅡ neutralization antibody were 90.12% and 91.35%,respectively.Conclusion It shows the vaccine was safe and the immunological efficacy was satisfied.