中国媒介生物学及控制杂志

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肾综合征出血热疫苗后备毒株的分离和生物学特性研究

姚苹苹;朱智勇;赵芝雅;朱函坪;徐芳;傅桂明;陆群英;翁景清;李敏红   

  1. 浙江省疾病预防控制中心病毒研究所 浙江省肾综合征出血热重点实验室 杭州310009
  • 出版日期:2004-10-20 发布日期:2004-10-20

Isolating of Vaccine Candidate Strain of Hemorrhagic Fever With Renal Syndrome and Its Characteristics

YAO Ping-ping; ZHU Zhi-yong; ZHAO Zhi-ya;et al   

  1. Institute for Virus Disease Research, Zhejiang Center for Disease Prevention and Control, Hangzhou 310009, China
  • Online:2004-10-20 Published:2004-10-20

摘要: 目的从肾综合征出血热(HFRS)疫区阳性鼠肺中分离汉坦病毒(HV),并对分离到的病毒进行型别鉴定和生物学特性研究,为HFRS疫苗后备毒种库的建立打下基础。方法疫区阳性鼠肺研磨液接种长爪沙鼠分离病毒,并将分离到的HV经乳沙鼠脑内连续传代,采用直接免疫荧光法和酶联免疫吸附试验,检查毒株的型别,研究传代后毒株的繁殖特性、病毒滴度和抗原滴度。结果从疫区32份阳性鼠肺中分离到9株HV,经单克隆直接荧光血清检查6株为汉滩病毒株,3株为汉城病毒株,经乳沙鼠脑内连续传代,其中4株毒株的病毒滴度和抗原滴度较高,汉滩和汉城毒株的病毒和抗原滴度最高分别达106.50、106.00和1∶5120、1∶5120。结论4株毒株的病毒滴度和抗原滴度均较高,可作为HFRS疫苗后备毒种的筛选毒株。

关键词: 汉坦病毒, 型别, 抗原性

Abstract: Objective Several vaccine candidate strains of hemorrhagic fever with renal syndrome(HFRS) were isolated from HFRS epidemic area,the sero type were identified and the antigenicity were studied.Methods The virus strains were continuously propagated in brain of sucking Mongolian gerbil.The characteristics of virus multiplication,viral titers and the amounts of virus antigen after serial passages were studied.Results 9 strains virus were isolated successfully from 32 positive mouse lung,of which 6 strains were determined as hantaan virus by Anti HFRS McAb and 3 strains as seoul virus.Virus titers and antigen titers of ZJ7(Hantaan virus,HTN) can reach 6.50 lgCCID50/ml、 1∶5 120,ZJ5(Seoul virus,SEO) and 6.00 lgCCID50/ml、 1∶5 120,respectively.Conclusion These two virus strain possessed high titers and good antigen and can be vaccine candidate strain of HFRS.