中国媒介生物学及控制杂志

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汉坦病毒感染Vero细胞病毒滴度和抗原滴度的动态观察

徐芳;姚苹苹;朱智勇   

  1. 浙江省疾病预防控制中心病毒研究所浙江省肾综合征出血热重点实验室 杭州300493
  • 出版日期:2004-08-20 发布日期:2004-08-20

Dynamic Change of the Hantavirus Titers and the Amounts of Virus Antigen in Vero Cells

XU Fang; YAO Ping-ping; ZHU Zhi-yong.   

  1. Institute for Virus Disease Research, The Center for Disease Prevention and Control of Zhejiang Province, Hangzhou 310009, China
  • Online:2004-08-20 Published:2004-08-20

摘要: 目的观察汉坦病毒(HV)在Vero细胞上的适应能力和毒株在该细胞中的增殖规律。方法将HV感染Vero细胞,每隔3d收取细胞培养液,直到细胞衰老,并观察细胞的病变情况。采用间接免疫荧光法(IFAT)和反向被动血凝试验(RPHA),观察细胞培养液的病毒滴度和抗原滴度的动态变化。结果HV不致Vero细胞产生病变,病毒感染细胞第8~11天时细胞培养液的病毒滴度和抗原滴度最高,以后逐渐降低,但直到细胞感染病毒32d时其培养液的病毒滴度和抗原滴度仍稳定在5.00LgTCID50/ml和1∶20以上。结论两株病毒已适应于Vero细胞,且具有病毒滴度高、毒力强和抗原性良好的特性,是Vero细胞肾综合征出血热灭活疫苗良好的后备筛选毒株。

关键词: 汉坦病毒, 细胞培养, 抗原性, 疫苗

Abstract: Objective To observe the adaptability of Hantavirus and the rule of these strains replication in Vero cells.Methods Virus are extracted every 3 days from Vero cells culture Hantavirus infected.Cytopathogenic effect(CPE) are observed until these cells degrade.Viral titers and the amounts of virus antigen after serial passages are observed by IFAT and RPHA.Results There was no cytopathogenic effect(CPE) in Vero cells after 32 days.Viral titers and the amounts of virus antigen reach to the top at 8-11th day after Hantavirus infect Vero cells.Viral titers and the amounts of virus antigen still keep up at the level of 5.00 Lg TCID50/ml and 1∶20 at 32th day after infaction.Conclusion The results suggest that these 2 candidate strains had adapted to Vero cells possessed high titers and good antigenicity and be feasible to prepare the HFRS vaccine in Vero cells.