关键词: 肠出血性大肠埃希菌O157∶H7, 鞭毛抗原, 抗-H7血清琼脂法

Abstract: Objective In order to evaluate anti-H7 serum agar medium assay for flagellar antigen of Enterohemorrhagic Escherichia coli O157∶H7.Methods 109 suspicious stains of O157∶H7 derived from feces of human,animal,food and water of enviroment were detected by anti-H7 serum agar medium assay for H7 flagellar antigen,in compare with tube agglutination test and the detection of species gene for H7,flagellor antigen positive and negative as control.Results The results showed that the three assay for detecting H7 flagellar antigen were good for the strains of positive and negative marker.The rate of sensitivity,specificity and coincident of anti-H7 serum agar medium assay were 100% at all in compare with tube agglutination test,and were 93.75%,95.31%,97.25% respectively in compare with detection of specific gene for H7.Conclusion The data showed that anti-H7 serum agar medium assay was a rapid,convenient and reliable method for detecting H7 flagellar antigen,so it was worthy to be used.