中国媒介生物学及控制杂志

• 论著 • 上一篇    下一篇

德国小蠊抗药性检测方法比较研究

曾晓芃;薛素琴;付学锋   

  1. 北京市疾病预防控制中心 北京100013
  • 出版日期:2004-02-20 发布日期:2004-02-20

Comparisons of Two Methods for Insecticide Resistance Detection to the German Cockroach,Blattella germanica

ZENG Xiao-peng; XUE Su-qin; FU Xue-feng   

  1. Beijing Center for Disease Prevention and Control, Beijing 100013, China
  • Online:2004-02-20 Published:2004-02-20

摘要: 目的对德国小蠊两种常用抗性检测方法进行比较,以了解不同方法在抗性系数(RR)上的差异,为根据需要选择适宜的抗性检测方法提供帮助。方法分别参照WHO和Atkinson等(1991)及Zhai等(1992)的方法。结果用药膜法测定KT50,德国小蠊对二氯苯醚菊酯、溴氰菊酯和氯氰菊酯的抗性倍数分别是6.93、13.85和5.60倍;而用点滴法测定LD50,德国小蠊对上述3种药剂的抗性倍数分别为22.17、64.32和27.03倍。可见,用点滴法比用药膜法测定德国小蠊对二氯苯醚菊酯、溴氰菊酯和氯氰菊酯的抗性系数分别要高出3.20倍、4.64倍和4.83倍。结论点滴法较药膜法更为灵敏,能较为准确地反映出德国小蠊的实际抗性水平,而药膜法相对简单易行,适于现场抗性检测。

关键词: 德国小蠊, 抗性检测方法

Abstract: Objective To compare difference of two methods for insecticide resistance detection in the resistance ratio of the German Cockroach,Blattella germanica.Methods Insecticide resistance was detected by methods of WHO and Atkinson & Zhai respectively.Results The resistance ratio(RR) measured by surface contact was 6.93 folds for permethrin,13.85 for deltamethrin and 5.60 for cypermethrin based on KT50.As measured by topical application,the resistance ratio based on LD50 was 22.17 folds for permethrin,64.32 for deltamethrin and 27.03 for cypermethrin.It means that the resistance ratios of this insect to permethrin,deltamethrin and cypermethrin by topical application were 3.20,4.64 and 4.83 times higher than that by surface contact method.Conclusion The results suggested that surface contact method do not accurately assess insecticides susceptibility in field populations of German cockroaches,topical application was more sensitive than surface contact tests for measuring insecticide resistance.