中国媒介生物学及控制杂志

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北京西山地区长角血蜱及其体内伯氏疏螺旋体实验观察

安京媛1;周国萍2;姚桂玲1;艾承绪3;李全民1   

  1. 1武警总医院 北京100039;2北京军区总医院;3军事医学科学院微生物流行病研究所
  • 出版日期:2002-08-20 发布日期:2002-08-20

Morphological Observation of Haemaphysalis congicornis and Borrelia Burgdorferi in the Tick's Bodyin Xishan Mountain in Beijing

AN Jing-yuan*; ZHOU Guo-ping; YAO Gui-ling; et al   

  1. General Hospital of the Armed Police,Beijing 100039,China
  • Online:2002-08-20 Published:2002-08-20

摘要: 目的:实验观察长角血蜱及其体内伯氏疏螺旋体形态结构。方法:1994~1996年在北京西山地区采集长角血蜱180只。标本应用蜱整体封片法、冰冻切片和石蜡切片结合HE染色和硝酸银染色法分别检测和光镜观察。结果:蜱整体封片法直接镜检即可观察到蜱外部基本形态结构。HE染色观察蜱内部器官细微形态结构。冰冻切片和石蜡切片结合硝酸银染色,检出北京西山地区长角血蜱(包括幼蜱、若蜱、成蜱和饱血蜱)伯氏疏螺旋体感染率为23%,显微镜下可清晰观察到蜱体伯氏疏螺旋体多种不同的形态。结论:上述方法对莱姆病媒介蜱及伯氏疏螺旋体的流行病学研究有一定价值。

关键词: 莱姆病, 长角血蜱, 伯氏疏螺旋体

Abstract: Objective:To observe the morpholosis of Haemaphysalis longicornis and histomorphological features of Borrelia burgdorferi(BB).Methods:Morpholosis of ticks may be observed by mounting method under light microscope.Morphology of the tick was observed by HE staining.Histomorphological features differentialy and morphology of BB was observed by frozed section and paraffin section combinend with sliver nitrate staining.Results:The Haemaphysalis longicornis were collected from 1994 to 1996 in Xishan Mountain in Beijing.180 individuals were examined.The infection rate of BB in ticks was 23% in the region.Conclusion:Three methods is useful for study and epidemic inspection of victor and BB of Lyme disease.