中国媒介生物学及控制杂志

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溴氰菊酯抗性家蝇酯酶的生化分析

曹晓梅;孙晨熹   

  1. 天津市卫生防病中心消毒与病媒生物控制学部 天津300011
  • 出版日期:2002-08-20 发布日期:2002-08-20

Biochemical Analysis of Esterase from Housefly(Musca domestica)Resistant to Deltamethrin

CAO Xiao-mei; SUN Chen-xi   

  1. Tianjin centers for Disease Control and Prevention, Tianjin 300011,China
  • Online:2002-08-20 Published:2002-08-20

摘要: 目的:研究拟除虫菊酯对家蝇的抗性机制。方法:通过对溴氰菊酯敏感、抗性和高抗性家蝇的离体酯酶活力测定、酶反应动力学及不连续非变性凝胶电泳,观察酯酶在拟除虫菊酯抗性中的作用。结果:用α-NA做底物酯酶活力,Vm在3种品系家蝇中无差异,2个抗性品系之间Km和Vm也无差异,但敏感品系的Km分别高于两抗性品系;聚丙烯酰胺凝胶电泳表明其同功酶谱差异很大,SS有6条带,R、RR各有5条带,且三者之间带的位置和带型均发生改变。结论:拟除虫菊酯抗性家蝇酯酶性质发生改变,可能参与对拟除虫菊酯的代谢抗性。

关键词: 家蝇, 拟除虫菊酯抗性, 酯酶

Abstract: Objective:To investigate the role of esterase in pyrethroid resistance of housefly.Methods:To detect the activity,kinetic parameters and the polyacryl gel electrophoresis spectrum of the esterase from the susceptible,resistant and super resistant strains.Results:The activity and the Vm of the esterase in the three strains were similar.The Km of the esterase from resistant and super resistant strains was also similar.But the Km of the esterase in the susceptible strain was much higher.Further polyacryl gel electrophoresis revealed that the esterase from different strains involves different isoenzyme composition.Conclusion:The changes of esterase isoenzymes is involved in the mechanisms for pyrethroid resistance in housefly.