中国媒介生物学及控制杂志

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杭州淡色库蚊抗性及酯酶活性测试

寇宇1;倪晓平1;乔传令2;李瑄2   

  1. 1杭州市疾病预防控制中心消毒监测与生物媒介防制所 浙江310006;中国科学院动物研究所
  • 出版日期:2002-02-20 发布日期:2002-02-20

ActiveTestofResistanceandEsteraseofHangzhouCulexpipienspallens

KOU Yu*; NI Xiao-ping; QIAO Chuan-ling; et al   

  1. Hangzhou Municipal Center For Disease Control & Prevention 310006,China
  • Online:2002-02-20 Published:2002-02-20

摘要: 目的:用淀粉电泳酯酶谱区别酯酶,检测淡色库蚊成蚊个体酯酶,并结合对幼虫的生物测试结果,探讨抗性生化机制及防制对策。方法:幼虫的生物测试采用浸渍法,成虫的非特异性酯酶测试采用淀粉电泳法。结果:淡色库蚊对氯氰菊酯抗性最高,是敏感品系的12.30倍;其后顺次为敌敌畏、高效氯氰、溴氰菊酯、残杀威、仲丁威,抗性倍数分别为5.61、5.09、4.65、4.43、3.98,胺菊酯、二氯苯醚菊酯、EBT、三氯杀虫酯抗性不明显。淀粉电泳结果蚊虫个体中存在过量产生的非特异性酯酶Estβ11和Estα2/β2,且蚊虫的酯酶谱较为复杂。结论:淡色库蚊对氯氰菊酯产生了中等抗性;其次对敌敌畏、高效氯氰、溴氰菊酯产生低抗性,宜换用药物。酯酶染色结果,存在引起有机磷抗性增高活性酯酶,酯酶谱且呈多态分布,与生物测试结果相一致,所以应减少敌敌畏的使用,注意轮换用药。

关键词: 淡色库蚊, 抗性, 酯酶活性, 生物测试

Abstract: Objective:To investigate the situation of the Hangzhou Culex pipiens pallens resistance,to differentiate esterase by using starch gel electrophoresis esterase flocculus and having biological test at the same time.Method:Dipping for instars and non-differential starch gel electrophoresis for imago.Results:The cypermethrin resistance was the highest one in six pesticides and it was 12.3 times of the sensitive strains.Dichlorphos,high-efficiency cypermethrin,deltamethrin,Propoxur,BPMC were respectively 5.61,5.09,4.65,4.43,3.98 times.The non-differential esterase Est β11 and Est α2/β2 had also come into being.Conclusion:Hangzhou Culex pipiens pallens produced middle resistance to cypermethrin and low resistance to other five insecticides.Some active esterases which have higher resistance to Organic phosphorus are in existence.