关键词: 肾综合征出血热, 灭活疫苗, 免疫增强, 防病效果, 免疫策略

Abstract: Objective:To go on observing the safety,immunology efficacy and protective efficacy of three different kinds of inactivated vaccines against HFRS:Type Ⅰ vaccines prepared from tissue culture of Mongolian gerbils kidney,type Ⅰ vaccines purified from mouse brains and type Ⅱ vaccines prepared from tissue culture of hamster kidney.Methods:The study combined field work and laboratory work.People in the study areas were randomized to vaccine group or control group.Antibody titers were tested by immunofluorescent antibody assay (IFA),Microcytopathic effect neutralization test (MCPENT),enzymelinked immunosorbent assay (ELISA) and hemagglutination inhibition test (HI).Results:These three kinds of vaccines were all safe.Antibody dependent immunization enhancement phenomenon was not observed among the inoculated population.Neutralizing antibody and immunofluorescent antibody were tested 14 days and 1 to 3 years after three doses of injection.The neutralizing antibody positive rate had declined to about 13.96% 1year after primary vaccination and increased to 73.49% 14 days after the booster dose (given 1 year after primary vaccination).At the third year after primary vaccination the neutralizingantibody positive rate declined to 27.81%.Similar changes of the immunofluorescent antibody positive rate were also observed.These three kinds of vaccines had remarkable preventive effect.The annual average protection rates were higher than 90% in all the 3 years after the primary vaccination.From these results,further suggestions on the immunology strategy were offered.