中国媒介生物学及控制杂志

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三种血清学方法对马来丝虫病监测的应用研究

王莉莉; 霍细香   

  1. 湖北省医学科学院寄生虫病研究所 武汉430079
  • 出版日期:1998-08-20 发布日期:1998-08-20

Applications of Three Serologic Tests for Surveillanceof Malayan Filariasisin Endemic Area

Wang Li-li; Huo Xi-xiang   

  1. Institute of Parasitic Diseases,Hubei Academy of Medical Sciences,Wuhan 430070
  • Online:1998-08-20 Published:1998-08-20

摘要: 目的:比较三种方法监测马来丝虫病的效果。方法:间接荧光抗体试验(IFAT)、酶联免疫吸附试验(ELISA)、斑点-酶联吸附试验(Dot-ELISA)。结果:检测1990、1991、1994年基本消灭马来丝虫病的流行区人群血清,IFAT阳性率分别为24.85%、20.32%和8.30%;ELISA阳性率分别为21.66%、30.32%和15.84%;Dot-ELISA阳性率分别为32.50%、10.96%和18.86%;总阳性率分别为46.66%、35.80%和32.07%。结论:以IFAT法较为稳定,3种方法联合应用,可提高阳性率。

关键词: 马来丝虫病, 血清学监测, 间接荧光抗体试验, 酶联免疫吸附试验, 斑点-酶联免疫吸附试验

Abstract: Application was conducted for detecting antibody levels of population in endemic area with Malayan filariasis(microfilarial rate being 0.08-0.72%) by using three serologic tests, IFAT,ELISA and Dot-ELISA in 1990, 1991 and 1994. The results showed that the positive rate were 24.58%, 20.32% and 8.3% with IFAT; 21.66%, 30.32% and 15.84% with ELISA; 32.5%,10.96% and 18.86% with Dot-ELISA; and the total positive rates were 46.66%, 35.8% and 32.07% respectively. The results showed that IFAT were found to be more stable. It was also proved that accumulative detection rate of using three tests be higher than each of the three tests.