中国媒介生物学及控制杂志

• 论著 • 上一篇    下一篇

再次从福建分离出斑点热群立克次体

陈振光1; 张健之2; 毕德增2; 陈敏2; 贺金荣2; 林荷1   

  1. 1福建省宁化县卫生防疫站365400; 2中国预防医学科学院流行病学微生物学研究所
  • 出版日期:1998-08-20 发布日期:1998-08-20

The Second Isolation of Spotted Fever Group Rickettsiae in Fujian

Chen Zhen-guang*; Zhang Jian-zhi; Bi De-zeng; et al   

  1. *Health and Anti-epidemic Station of Ninghua County, Fujian Province 365400
  • Online:1998-08-20 Published:1998-08-20

摘要: 目的:从蜱类媒介中分离斑点热群立克次体。方法:用鸡胚卵黄囊培养法直接分离。结果:自福建省宁化县林区麂体表捉获的越原血蜱(Haemephysalis yeni)中分离到1株斑点热群立克次体,经微量补结试验和PCR检测初步确定为西伯利亚立克次体(Ricketsia sibirica)。结论:进一步从病原学上证明了福建省存在北亚斑点热自然疫源地的可能性。

关键词: 立克次体分离, 微量补结, PCR, 越原血蜱

Abstract: Objectives: To isolate spotted fever group rickettsiae from tick vectors. Methods: Directly isolated rickettsiae using embryonated hen's eggs. Results: A strain of spotted fever group rickettsiae was isolated from Haemaphysalis yeni collected from Muntiacus reevesi Ogil by captured in a forest area of Ninghua county, Fujian province. The isolate was primarily identified as Rickettsia sibirica by micro-complement-combination assay, polymerase chain reaction and other molecular biological techniques. Conclusion: These data provide more aetiological evidence for existence of North Asia Fever in Fujian province.