中国媒介生物学及控制杂志

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广东首次分离到莱姆病螺旋体

陈戊申1; 万康林2; 郝瑞丰1; 何剑峰1; 何立3; 罗会明1; 曾汉武1; 张金声2; 张哲夫2   

  1. 1广东省卫生防疫站 广州510300; 2中国预防医学科学院流行病学微生物学研究所; 3连平县卫生防疫站
  • 出版日期:1998-08-20 发布日期:1998-08-20

Borrelia burgdorferi was Isolated in Guangdong

Chen Wu-shen*; Wan Kang-lin; Hao Rui-feng; et al   

  1. Health and Anti-epidemic Station of Guangdong Province
  • Online:1998-08-20 Published:1998-08-20

摘要: 目的:分离莱姆病螺旋体。方法:鼠、蜱标本接种BSK培养基,分离病原体与单克隆抗体进行间接免疫荧光试验。结果:用BSK培养基分离49只野鼠和250匹蜱,从1只白腹巨鼠胚胎分离到1株螺旋体(LPR30)。结论:鉴定为莱姆病螺旋体。

关键词: 莱姆病螺旋体, 白腹巨鼠, 动物宿主

Abstract: A survey of animal reservoir of Lyme disease was conducted in June,1996.49 mice and 250 ticks were tested by isolation using BSK medium.A strain of spirochete (LPR30) was isolated from the embryo of rat (Rattus edwerdsi).Characterization of the spirochete by microscopy and serology (IFA) using genusand speciesspecific monoclonal antibodies (H5332、H9724、H6831),confirmed that it is a strain of Borrelia burgdorferi. 