中国媒介生物学及控制杂志

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胺菊酯选育淡色库蚊抗性株的研究

朱礼华; 赵彤言; 董言德; 于虹; 陆宝麟   

  1. 军事医学科学院微生物流行病研究所 北京100071; #山西省卫生防疫站访问学者
  • 出版日期:1998-04-20 发布日期:1998-04-20

Study on Resistant strain of Culex pipiens pallens Selecting with Tetramethrin

Cheng Jing-xia; Zhu Li-hua; Zhao Tong-yan; et al   

  1. Institute of Microbiology & Epidemiology,AMMS,Beijing,100071
  • Online:1998-04-20 Published:1998-04-20

摘要: 目的:实验室选育淡色库蚊胺菊酯抗性品系。方法:LC50测定采用浸液法,选育工作参照Omer SM et al方法。结果:1996年连续用药筛选6代,抗性倍数在第4代曾达到14倍,尔后种群数量逐渐衰落,至第6代未能继续传代。1997年重新选育,共连续用药筛选8代,前3代抗性水平逐步上升,达13.07倍;之后逐代下降,第6代降为4.57倍,第7代有所回升,第8代又有降低。同时进一步研究抗性种群的吸血率、产卵率、各虫期的存活率等种群生存数据发现,与敏感品系相比抗性种群各值均有所下降,种群适合度有一定程度降低,相对适合度为0.29。结论:本实验中抗胺菊酯品系抗性上升缓慢,可能是胺菊酯的作用机理及其作为击倒剂的作用特点所致。至于抗性种群有无遗传分化,尚需进一步研究。

关键词: 淡色库蚊, 胺菊酯, 抗药性选育

Abstract: Culex pipiens pallens was selected successively for 8 generations with tetramethrin in laboratory.The LC50 of selected strain increased from 0.140 ppm to 1.829ppm after 3 generations of selection (13.07 folds).Then the LC50 went down every generation.The LC50 is only 0.643ppm (4.57 folds) at the 6th generation.After that increase of LC50 is not steady.Some biological parameters were compared between S strain and R strain,It was found that the rates of bloodfeeding and surviving in larvae and pupae of the 8th generation of R strain declined.Relative fitness by net reproductive rates (R0) was 0.29.