肾综合征出血热疫苗效果免疫(感染)增强和免疫策略研究

摘要/Abstract

摘要： 目的：观察我国1993～1994年试生产的肾综合征出血热（HFRS）沙鼠肾细胞Ⅰ型灭活疫苗（上海生研所和杭州天元公司生产）、乳鼠脑纯化Ⅰ型灭活疫苗（兰州生研所生产）、地鼠肾细胞Ⅱ型灭活疫苗（长春生研所生产）的安全性，考核血清学、近期和中期流行病学效果。方法：采用IFAT法检测特异性IgG抗体，用MCPENT法检测中和抗体滴度。结果：3种疫苗安全性皆较好，上海沙鼠苗、天元沙鼠苗、鼠脑苗和地鼠苗中强反应率分别为0.56％、0．03％、3.26％、1.57％。3种疫苗3针全程免疫后14天、加强前、加强后14天和1年，中和抗体阳转率平均为：地鼠苗78．36％、12.33％、80．56％和54．46％，上海沙鼠苗57．81％、10.79％、70．71％和35.58％，天元沙鼠苗70.00％、50．00％、91．18％和未到期，鼠脑苗51．09％、10.48％、61．90％和45.00％；荧光抗体阳转率地鼠苗为65．73％、33．90％、85．00％和49.44％，上海沙鼠苗93．69％、17．76％、89．04％和51.61％，天元沙鼠苗83．33％、12.90％、64．52％和未到期，鼠脑苗84．27％、12.50％、81.25％和0％。3种疫苗近期（基础免疫后1年）和中期（基础免疫后2年）流行病学防病效果均较好，其平均保护率：地鼠苗分别为97．81％、88．73％，上海沙鼠苗94.08％、92.17％，天元沙鼠苗100.00％、100.00％，鼠脑苗88.45％、100.00％。试区内疫苗接种者再感染发病与未接种者感染发病的病情轻重无明显差别；接种者在流行高峰期后抗体阳转或增高者（视为再感染）均无症状。目前我们尚未发现接种人群中有免疫（感染）增强问题。结论：3种疫苗近期（基础免疫后1年）和中期（基础免疫后2年）流行病学防病效果均较好，并对免疫策略提出建议。

关键词: 肾综合征出血热, 灭活疫苗, 预防效果, 免疫增强, 免疫策略

Abstract: Objective: The purpose of this report was to observe the safety, serological efficacy, shortterm and long-term epidemiological efficacy character of three different kinds of inactivated vaccines against HFRS (Type l vaccines purified from tissue culture of Mongoian gerbils kindney, supplied by Institute of Biological Preducts, Shanghai and Tianyuan Cwnpany; type I vaccines purified from mouse brains supplied by Institute of BioIogical Products, Lanzhou; type IIvaccines prepared from tissue culture of hamster kidney, supplied by Institute of Bidogical Products, Changchun), which pr0cluced from 1993to 1994 in China.Methods used in antibody test: Immunofluorescent antithey assay was used in testing specific IgG antithey and McrcaCPE methed was used in testing the titer of neutralizing antidriy.Results:The three kinds of vaccines were all safe.The moderate and severe side effects of them were 0.56%,0.03%, 3.26% and 1.57% respectively.14 dayS after three doses of injection, before reinforced, 14 days and 1 year after reinforced.The rates of the rates of serocoversion of neutralizing antibody by the Mcro-CPE methed were 78.36%, l2.33%, 8O.56% and 54.46% for hamster kidney vaccines57.81%, 10.79%, 70.71% and 35.58% for Shanghai Mongoian gerbils kidncy vaccines;7O.00%, 50.00%, 91.18% and unknown (not yet completed) for Tianpon MOngian gerbils kidney vaccines; 51.O9%, 10.48%, 61.90% and 45.00% for mouse brains vaccines.The rates of seroconversion of immunonuorescent antibody by IFA method were 65.73%, 33.90%, 85.00% and 49.44% for hamster kidney vaccines; 93.69%, 17.76%, 89.04% and 51.61% for Shanghai Wgoian gErbils kidney vaccines;83.33%, 12.90%, 64.52% and unknown (not yet completed) for TianyUall Mongoian gerbils kidney vaccines; 84.27% , 12.50%, 81.25% and 0% for mouse brains vaccines.According to the short-term (1 year after primary immunization) and middle-term (2 years after primary immunizatbo)observation, these three kinds of vaccines were effective, and their protection rates in population were 97.81%, 88.73% for hamster kidney vaccines; 94.08%, 92.17% for Shanghai Mongoian gerbils kidney vaccines; 100.00%, 100.00% for Tianyuan Mongolian gerbils kidney vaccines; 88.45%, 100.00%for mouse brain vaccines.There was no significant difference in clinical manifestation between the inoculated and un-inoculated people after another infection.After the epidemic peak, the inoculated individuals didn't show any clinical symptom while had seroconversion or increase of antithey titer which could be defined as second infection.Till now, we haven't find any antibody dependent immunhatbo enhancernent phenomenon among the inoculated Population.Conclusion: According to the short-term and middle-term (1 or 2 years after primary immunization) observation, these three kinds of vaccines were all ePidendologically effective.We also offered some suggestions on the immunofogcal strategy.

“九五”国家医学科技攻关96－906－03－13专题协作组. 肾综合征出血热疫苗效果免疫(感染)增强和免疫策略研究[J]. 中国媒介生物学及控制杂志.

Cooperative Group on the Ninth-Five Years National Medical Scientific Study 96-906-03-13. Evaluation on the Efficacy of Vaccines against HFRS and Study on their Antibody Dependent Immunization Enhancement and Immunological Strategy[J]. Chines Journal of Vector Biology and Control.

[1]	周济华, 张云智, 章域震, 杨卫红, 冯云. 云南省2012－2020年肾综合征出血热流行特征分析[J]. 中国媒介生物学及控制杂志, 2021, 32(6): 715-719.
[2]	李皓晨, 齐滢滢, 张翀, 韩文菊, 沈铁峰, 李德强, 关鹏, 黄德生. 基于贝叶斯网络的肾综合征出血热发病率预测模型研究[J]. 中国媒介生物学及控制杂志, 2021, 32(4): 475-480.
[3]	刘菁, 陈阳, 林代华, 肖方震, 林文, 王加熊, 韩腾伟, 刘维俊. 福建省2016－2018年肾综合征出血热流行特征和宿主动物监测分析[J]. 中国媒介生物学及控制杂志, 2021, 32(3): 329-333.
[4]	刘天, 夏世国, 梅芳盛, 郝海波, 聂晓培, 黄继贵, 姚梦雷, 刘力, 杨瑞, 胡兵, 吴杨, 童叶青, 陈琦. 湖北省荆州市2017－2018年肾综合征出血热宿主动物监测结果分析[J]. 中国媒介生物学及控制杂志, 2020, 31(4): 469-473.
[5]	胡挺松, 胡秋凌, 李绍雄, 黄勇, 胡海梅, 高丽芬, 罗琼梅, 何永芝, 周永存, 张海林, 张富强. 云南省楚雄市2015-2018年肾综合征出血热流行特征及宿主动物调查[J]. 中国媒介生物学及控制杂志, 2020, 31(2): 152-157.
[6]	庞卫龙, 郑翔, 葛君华, 崔清荣, 刘营, 胡雅飞, 沈伟伟, 胡海燕, 丁斌彬, 孙继民, 林海江. 浙江省天台县1984-2018年肾综合征出血热监测分析[J]. 中国媒介生物学及控制杂志, 2020, 31(2): 158-163.
[7]	张洁, 王子江, 李鑫, 刘学升, 刘芸, 孙英伟, 姚文清. 辽宁省2012－2016年肾综合征出血热监测结果分析[J]. 中国媒介生物学及控制杂志, 2019, 30(6): 626-629.
[8]	陈阳, 邓艳琴, 林代华, 肖方震, 林文, 王加熊, 韩腾伟, 刘菁, 刘维俊. 福建省部分地区首次确定肾综合征出血热疫源地的调查研究[J]. 中国媒介生物学及控制杂志, 2019, 30(6): 692-695.
[9]	彭珵, 胡祝敏, 李艳君, 关鹏, 黄德生. 基于微分动力学方程的沈阳市肾综合征出血热疫情模拟研究[J]. 中国媒介生物学及控制杂志, 2019, 30(5): 498-501.
[10]	李红兵, 田辉, 付海龙, 何微, 胡晓倩, 高娟, 严钏元. 陕西省宝鸡市肾综合征出血热时空聚集性分析[J]. 中国媒介生物学及控制杂志, 2019, 30(5): 506-509.
[11]	孙琬琬, 刘小波, 吴海霞, 王君, 刘起勇, 鲁亮. 基于分布滞后非线性模型的葫芦岛和丹东市环境温度对肾综合征出血热的效应研究[J]. 中国媒介生物学及控制杂志, 2019, 30(3): 272-277.
[12]	胡泉博, 陈淑红, 孙巍, 许军, 华华, 杨明, 李冀宏, 周广恩. 黑龙江省2014－2016年肾综合征出血热宿主动物疫情监测研究[J]. 中国媒介生物学及控制杂志, 2019, 30(3): 278-280.
[13]	付仁龙, 陈盛恩, 夏金连, 柳小青, 吴亚云, 郑卫青. 南昌市安义县2009－2016年肾综合征出血热宿主动物监测结果分析[J]. 中国媒介生物学及控制杂志, 2019, 30(2): 200-202.
[14]	刘天, 黄继贵, 郝海波, 李斌, 刘漫, 刘力, 夏世国, 毛安禄. 湖北省荆州市2017年肾综合征出血热流行特征及宿主动物调查[J]. 中国媒介生物学及控制杂志, 2018, 29(6): 609-612.
[15]	王妍, 魏亚梅, 韩旭, 韩占英, 蔡亚男, 齐顺祥, 李琦. 河北省肾综合征出血热疫区鼠类构成及其携带汉坦病毒调查[J]. 中国媒介生物学及控制杂志, 2017, 28(6): 553-556.

肾综合征出血热疫苗效果免疫(感染)增强和免疫策略研究

Evaluation on the Efficacy of Vaccines against HFRS and Study on their Antibody Dependent Immunization Enhancement and Immunological Strategy

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价