关键词: 亚洲璃眼蜱, 基因组, 多态DNA聚合酶链技术

Abstract: We reported the study on polymorphism DNA of Boophilus microplus with RAPD-PCR technique in the first instance.Here,we amplified the genomic polymorphism DNA maps of Hyaloma asiaticum asiaticum with three primers-“P_z” (CGGCCCGGTA),“P_w”(CGGCCCCGGT) and“P_y”(CTGGAGGACT).The molecular weights (bp) of the DNA bands for each map were:“P_z”: 1380,1230,955,912,871,794,741,668,646,562, 537,442; "P_w":955,724,668,562,537,457,279; "P_y":1072,912,832,794,612,582,562,537,422,385. The RAPD-PCR for its simplicity, rapidness,and being able to reveal difference and evolutional relationships at the level of DNA,could be developed into an ideal taxonomic method for Acarology.