中国媒介生物学及控制杂志

• 论著 •    下一篇

不同诱导源诱导美洲大蠊血淋巴抗菌物质的研究

龚琪; 孟阳春; 周洪福   

  1. 苏州医学院寄生虫学教研室 苏州215007
  • 出版日期:1993-04-20 发布日期:1993-04-20

Studies on Antibacterial suBstances from the Haemolymph of periplaneta americana Inducedby Several factors

Gong Qi;et al   

  1. Department of Parasitology Suzhou Medical College, Suzhou 215007
  • Online:1993-04-20 Published:1993-04-20

摘要: 60Co γ射线、活的大肠杆菌(Escherichia coli)及热灭活的金黄色葡萄球菌(Staphylococcus aureus)、绿脓杆菌(pseudomonas aeruginosa)和生理盐水,均能诱导美洲大蠊(Periplaneta americana)血淋巴中的抗菌物质,但不同诱导源诱导产生抗菌物质的活性大小不同,达到最大活性的时间不同,抗菌特性亦有差异。经热灭活细菌诱导的抗菌物质抗菌活性大,抗菌种类多,而且对诱导之细菌的抗菌效果还有一定的针对性,即对该菌的杀菌效果比对其它细菌强,其中抗绿脓杆菌和伤寒杆菌(Salmonella tuphi)的作用比庆大霉素(10μg/片)和氯霉素(30μg/片)抗菌素纸片的抗菌作用强。

关键词: 美洲大蠊, 血淋巴, 抗菌物质

Abstract: Periplaneta americana could be induced to produce antibacterial substances in the haemolymph by injection with live E. coli, heat killed S. aureus, P. aeruginosa and saline or treatment with 60Co γ-rays, and with different respones. The haemolymph from the insect vaccinated by heat killed S. aures and P. aeruginosa was more active against bacteria than that by live E. coli or treated with 60Co γ-rays. It was found that the haemolymph was efficient against several Grampositive and Gram-negative bacterial strains. It was also found that the haemolymph activity against P. aeruginosa and S. typhi was stronger than the antibacterial activity of the pieces of paper contained 10μg gentamicin or 30μg chloramphenicol, respectively.