中国媒介生物学及控制杂志

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自殖性东乡伊蚊卵黄发生的初步研究

孙建新; 谭璟宪   

  1. 第二军医大学寄生虫学教研室 上海200433
  • 出版日期:1992-06-20 发布日期:1992-06-20

Preliminary Study of vitellogenesis in Autogenous Mosquito,Aedes toqoi

Sun Jianxin; Tan Jingxian   

  1. Department of Parasitology, Secind Military Medical University. Shanghai 200433
  • Online:1992-06-20 Published:1992-06-20

摘要: 本文首次报道用组织化学、免疫组织化学和免疫电泳等方法,研究东乡伊蚊自殖株卵黄发生过程中脂肪体合成卵黄原蛋白及其动态和血淋巴内卵黄原蛋白含量动态的实验结果。与同种蚊虫非自殖株的实验结果对比,发现自殖株蚊虫脂肪体细胞合成卵黄原蛋白在羽化后即启动,羽化初脂肪体细胞质内有密集的卵黄原蛋白颗粒,合成高峰见于羽化后24h,至36h开始下降,60h之脂肪体细胞内这种蛋白颗粒很稀少;卵母细胞的变化情况与此相反,随着脂肪体细胞内蛋白颗粒密度的下降,卵母细胞内的卵黄蛋白颗粒密度增加,并逐步发育成熟。非自殖株蚊虫血食前脂肪体细胞和卵母细胞发育有明显的休止期,合成卵黄原蛋白始于血食后,并于24h达高峰:卵母细胞的情况与自殖株蚊虫相似。实验还证实,无脂肪体外组织合成卵黄原蛋白。自殖株蚊虫血淋巴内卵黄原蛋白在羽化初就有一定浓度,羽化后36h达高峰,随后即下降;有非殖株血淋巴内卵黄原蛋白浓度高峰在血食后24h。上述结果表明,东乡伊蚊同种不同生理株的卵黄发生虽其过程一致,但其生物学特征各有不同,在脂肪体细胞合成卵黄原蛋白的启动时间,合成能力及卵黄原蛋白在血淋巴中的动态等方面存在差异。

关键词: 东乡伊蚊, 卵黄发生, 自殖, 血淋巴

Abstract: This article firstly reports the experimental results of vitellogenin(YG)synthesis by fat body cells(FBCs)and VG concentration in hemolymph at different times during the period of vitellogenesis in autogenous Aedes togoi. Comparing with the results of an unautogenous strain of the same species, it was found that VG synthesis of autogenous strain started quickly after emergence. The cytoplasm of FBCs was filled with VG granaules in early time post-emergence. The peak of VG synthesis was at 24th hr and declined at 36th hr.There was hardly any VG granule in FBCs at 60th hr. With VG granuals in FBCs decreasing, the density of yitelline (Vn) granuales in oocytes increased and so oocytes developed gradually and continuously to maturation. There was a marked resting stage during the period of vitellogenesis of anautogenous strain. The VG synthesis was initiated only if feeding blood meal, and its peak was at 24th hr. The developmental matters of the oocytes were similar to that of autogenous strain. The results also demonstrated that no other tissues synthesized VG except FBCs. There were a little VG levels in early time post-emergeuce in hemolymph of autogenous strain. The peak of VG concentration was at 36th hr, and then decreased. The peak of VG concentration in henolymph anautogenous strain was at 24th hr after blood meal.It is suggested by mentioned results that the processes of vitellogenesis in different physiological strain of the same species in Aedes togoi is similar, but the biological characteristics of vitellogenesis are different. There exists differences in initiating time and eapacity of VG synthesis by FBCs and VG concentration in hemolymph at different time.