淡色库蚊细胞早熟凝聚染色体的诱导

中国媒介生物学及控制杂志

淡色库蚊细胞早熟凝聚染色体的诱导

赵郁光; 蓝明扬

江苏省寄生虫病防治研究所无锡214064

出版日期:1992-06-20 发布日期:1992-06-20

Induction of Premature Chromosome Condensation in Mosquito Cells from Culex pipiens pallans

Zhao Yuguang; Lan Mingyang

Jiangsu Institute of Parasitic Diseases

Online:1992-06-20 Published:1992-06-20

摘要/Abstract

摘要： 用淡色库蚊建立的细胞系(Cpp512)细胞与中国仓鼠卵巢细胞融合,诱导出蚊细胞的早熟凝聚体(PCC)。融合剂使用分子量为4000的50%聚二乙醇(PEG),有丝分裂细胞与间期细胞比例为1;15,在32℃下融合2～3min,温育时间为1h。早熟凝聚染色体分三种基本类型。G₁-PCC为单股,早期比晚期浓缩;S-PCC呈“粉碎”样外观;G₂-PCC为双股,分散好,数目可数,类似于有丝分裂期染色体,但更长,故可用作高分辨显带及其它细胞遗传学的进一步研究。PCC技术可成为实用有效的蚊细胞遗传学研究技术。

关键词: 淡色库蚊细胞, 早熟凝聚染色体

Abstract: Premature condensed chromosome (PCC) of mosquito cells (Cpp512 cell line, established from Culex pipiens pallans)was induced by fusing with mitotic Chinese Hamster Ovary(CHO) cells, using 5)% polyethylene glycol(PEG, MW=4000)as fusogen. The ratio of the mitotic cells to interphase cells was 1: 15. The fusion was performed at 32℃ for 2-3 min., then incubated for 1 h. The PCC morphology clearly showed 3 kinds of essential types: G₁-PCC had single chromatid, the early phase was more condensed than later one: S-PCC had a "pulverized" appearance; G2-PCC had had donble chromatids which were well spread and countable, just like the mitotic chromosome but much longer than the mitotic one, G₂-PCC therefore could be used for high resolution chromosome studies. The PCC technique would be useful and powerful tools in mosquito cytogenetic study.

赵郁光; 蓝明扬. 淡色库蚊细胞早熟凝聚染色体的诱导[J]. 中国媒介生物学及控制杂志.

Zhao Yuguang; Lan Mingyang. Induction of Premature Chromosome Condensation in Mosquito Cells from Culex pipiens pallans[J]. Chines Journal of Vector Biology and Control.