关键词: 尖音库蚊复组, 成虫蛋白, 聚丙烯酰胺凝胶电泳, 薄层等电聚焦

Abstract: Protein component of Culex pipiens complex from zhenjiang, Qingdao, Jinan and Yantai in four geographic strains has been studied by polyaerylamide gel electrophoresis(PAGE). At the Same time, we compared further adult proteins of Cx. pipiens pallens from Jinan and Yantai by thin-layer isoelectric focusing(IEF). The results are as follows: As a whole, no significant difference was observed among the mosqiutoes in electrophic bands of the adult proteins. There were some particularities in the prorein electrophoresis scanning figures of Cx. pipiens pallens from Qingdao, Cx. pipiens quinquefasciatus and intermediate form from Zhenjiang. The phenomena as mentioned above were closely related to the ecologic environment and genetic gene respectively, but their prolein electrophoresis bands were all similar. Therefore, the differences among them didn't exceed subspecies level for four geographic strains of Cx. pipiens complex. in addition, thin-layer IEF technique is better than PAGE method. As the former is worth the popularization.