紫外分光光度法测定苏云金杆菌187菌株伴胞晶体蛋白的应用研究

中国媒介生物学及控制杂志

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紫外分光光度法测定苏云金杆菌187菌株伴胞晶体蛋白的应用研究

刘军; 徐博钊

湖北省医学科学院寄研所武昌430070

出版日期:1991-12-20 发布日期:1991-12-20

Study on determining crystal protein Content of Bacillus thuringiensis by ultraviolet spectrophotometry

Liu Jun;Xu Bozhao

Institute of parasitic disease Hubei Academy of Medical sciences,Wuchang,430070

Online:1991-12-20 Published:1991-12-20

摘要/Abstract

摘要： 用紫外分光光度法测定187菌株的晶体蛋白含量,借此推算其制剂的效价,并将此法与常规的生物测定法进行平行比较,发现两者所得结果的符合率达90%以上,紫外分光光度法具有更为简便、快速等突出优点。

关键词: 紫外分光光度法, 伴胞晶体蛋白, 碱溶曲线, 差速离心

Abstract: The crystal protein content of B. thuringiensis(strain-187)was determined by ultraviolet spectrophotometry, and its corresponding titration has been compared with that by routine bioassay technique. (According to determing their titration unit against insect were estimated.) The results showed that the datum from two of the techniques were very close, and ultraviolet spectrophotometry is more simple, quicker and practical than the others.

刘军; 徐博钊. 紫外分光光度法测定苏云金杆菌187菌株伴胞晶体蛋白的应用研究[J]. 中国媒介生物学及控制杂志.

Liu Jun;Xu Bozhao. Study on determining crystal protein Content of Bacillus thuringiensis by ultraviolet spectrophotometry[J]. Chines Journal of Vector Biology and Control.

紫外分光光度法测定苏云金杆菌187菌株伴胞晶体蛋白的应用研究

Study on determining crystal protein Content of Bacillus thuringiensis by ultraviolet spectrophotometry

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