中国媒介生物学及控制杂志 ›› 2025, Vol. 36 ›› Issue (2): 251-257.DOI: 10.11853/j.issn.1003.8280.2025.02.018

• 调查研究调查研究 • 上一篇    下一篇

2023年云南省景洪市登革热流行季节媒介伊蚊种群构成及虫媒病毒感染调查

王雪菲1, 陈丽2, 谭力滔3, 邹建红3, 姜进勇1   

  1. 1. 云南省热带传染病国际联合实验室, 云南省病媒生物学与控制国际联合实验室, 云南省虫媒传染病防控重点实验室, 云南省虫媒传染病防控关键技术创新团队, 云南省寄生虫病防治所, 云南 普洱 665000;
    2. 昆明理工大学 生命科学与技术学院, 云南 昆明 650000;
    3. 景洪市疾病预防控制中心, 云南 景洪 666100
  • 收稿日期:2024-08-14 出版日期:2025-04-20 发布日期:2025-05-09
  • 通讯作者: 姜进勇,E-mail:yipdjiang@126.com
  • 作者简介:王雪菲,女,硕士,主管医师,主要从事虫媒病毒性疾病防控及病原检测相关科研工作,E-mail:yipdwxf@163.com;陈丽,女,在读硕士,主要从事微生物学与病媒控制研究,E-mail:chenli20210325@163.com
  • 基金资助:
    云南省科技人才与平台计划(202305AC160012);科技基础资源调查专项(2022FY100900)

Investigation of the population composition and arbovirus infection of Aedes vector during dengue fever epidemic season in Jinghong, Yunnan Province, China, 2023

WANG Xue-fei1, CHEN Li2, TAN Li-tao3, ZOU Jian-hong3, JIANG Jin-yong1   

  1. 1. Yunnan International Joint Laboratory of Tropical Infectious Diseases, Yunnan International Joint Laboratory of Vector Biology and Control, Yunnan Provincial Key Laboratory of Vector-borne Diseases Control and Research, Yunnan Institute of Parasitic Diseases, Pu'er, Yunnan 665000, China;
    2. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650000, China;
    3. Jinghong Center for Disease Control and Prevention, Jinghong, Yunnan 666100, China
  • Received:2024-08-14 Online:2025-04-20 Published:2025-05-09
  • Supported by:
    Yunnan Province Science and Technology Talents and Platform Plan (No. 202305AC160012); Science and Technology Basic Resources Investigation Project (No. 2022FY100900)

摘要: 目的 调查2023年云南省西双版纳傣族自治州(西双版纳州)景洪市登革热疫情暴发期间白纹伊蚊和埃及伊蚊种群构成及虫媒病毒感染情况,为登革热等虫媒病毒性疾病防控提供科学依据。方法 2023年景洪市登革热疫情暴发期间(8-10月),采用双层叠帐法监测城区白纹伊蚊和埃及伊蚊成蚊种群构成变化情况并采集伊蚊样本,采用实时荧光定量PCR(qPCR)法检测蚊虫登革病毒、寨卡病毒和基孔肯雅病毒感染情况,反转录PCR(RT-PCR)法扩增病毒E基因序列并进行溯源分析。结果 2023年8-10月共采集到成蚊730只,埃及伊蚊548只(75.07%),白纹伊蚊182只(24.93%)。730只伊蚊根据种属、雌雄、采集时间和地点共分为76组(白纹伊蚊21组,埃及伊蚊55组),共11组(14.47%)检出登革热病毒1型(DENV-1),白纹伊蚊感染率为9.52%(2/21),埃及伊蚊为16.36%(9/55),2种伊蚊感染率差异无统计学意义(χ2=0.574,P=0.449);未发现携带寨卡病毒和基孔肯雅病毒的伊蚊。从11组DENV-1阳性样本中成功获得登革病毒E基因序列4条,1条来自白纹伊蚊,3条来自埃及伊蚊,4株DENV-1核苷酸相似性为100%,均属Genotype Ⅰ基因型。系统进化分析表明4株DENV-1和2023年景洪株、2023年广州株、2019年老挝株和2019年缅甸株高度同源。结论 景洪市登革热流行季节埃及伊蚊较白纹伊蚊分布广泛,2种伊蚊均携带登革1型病毒,应加强伊蚊感染虫媒病毒调查和研究工作。

关键词: 登革病毒, 寨卡病毒, 基孔肯雅病毒, 伊蚊, E基因, 进化分析, 景洪市

Abstract: Objective To investigate the population composition and arbovirus infection of Aedes aegypti and Ae. albopictus in Jinghong, Xishuangbanna Dai Autonomous Prefecture (Xishuangbanna Prefecture), Yunnan Province, China during the dengue fever epidemic season in 2023, so as to provide a basis for the prevention and control of dengue fever and other arboviral diseases. Methods During the dengue fever epidemic season in 2023 (from August to October), the change of population composition of Ae. aegypti and Ae. albopictus was monitored in urban areas and Aedes mosquitoes were collected using the double mosquito net method. The infections with Dengue virus, Chikungunya virus, and Zika virus were detected using quantitative PCR (qPCR). The E gene sequences of the viruses were amplified using reverse transcription-PCR (RT-PCR), and a phylogenetic analysis was performed for homological analysis. Results A total of 730 Aedes mosquitoes were collected, of which 548 (75.07%) were Ae. aegypti and 182 (24.93%) were Ae. albopictus. All mosquitoes were divided into 76 groups (21 groups for Ae. albopictus and 55 groups for Ae. aegypti) based on species, sex, and time and location of acquisition. Out of the 76 groups, 11 (14.47%) tested positive for Dengue virus type 1 (DENV-1), yielding an infection rate of 9.52% (2/21) for Ae. albopictus and an infection rate of 16.36% (9/55) for Ae. aegypti, with no significant difference between the two species (χ2=0.574, P=0.449). Zika virus and Chikungunya virus were not detected in any of the mosquitoes. Four DENV-1 E gene sequences (1 from Ae. albopictus and 3 from Ae. aegypti) were successfully obtained from the 11 groups of DENV-1 positive specimens. The sequence similarity of the four sequences was 100%, and all sequences belonged to genotype Ⅰ. The phylogenetic analysis revealed that the strains were closely related to the 2023 Jinghong strain, 2023 Guangzhou strain, 2019 Laos strain, and 2019 Myanmar strain. Conclusions Ae. aegypti is more widely distributed than Ae. albopictus in Jinghong during the dengue fever epidemic season, and DENV-1 is detected in both species. Investigation and research should be enhanced for arboviral infection with Aedes mosquitoes.

Key words: Dengue virus, Zika virus, Chikungunya virus, Aedes, E gene, Phylogenetic analysis, Jinghong

中图分类号: