2020年新疆霍尔果斯口岸地区鼠类及其体表寄生虫携带病原体调查

doi:10.11853/j.issn.1003.8280.2022.06.026

中国媒介生物学及控制杂志 ›› 2022, Vol. 33 ›› Issue (6): 906-911.DOI: 10.11853/j.issn.1003.8280.2022.06.026

2020年新疆霍尔果斯口岸地区鼠类及其体表寄生虫携带病原体调查

田锋¹, 耿合员², 夏侯为民³, 张子龙⁴, 郭强⁵, 赵丹云⁵, 孙玉婷¹, 史燕¹, 蔡晓雪¹, 陈超怡¹, 蒋璐¹, 程慧¹

1. 新疆国际旅行卫生保健中心(乌鲁木齐海关口岸门诊部)医学检验科, 新疆乌鲁木齐 830011;
2. 江苏国际旅行卫生保健中心(南京海关口岸门诊部), 江苏南京 210000;
3. 韶关海关综合技术服务中心技术部, 广东韶关 512023;
4. 上海国际旅行卫生保健中心(上海海关口岸门诊部), 上海 200050;
5. 天津国际旅行卫生保健中心 (天津海关口岸门诊部), 天津 300456

收稿日期:2022-05-25 出版日期:2022-12-20 发布日期:2022-12-09
通讯作者: 夏侯为民,E-mail:376773792@qq.com;耿合员,E-mail:379549257@qq.com
作者简介:田锋,男,硕士,副主任技师,从事口岸传染病病原生物学、分子病毒学、流行病学及疫苗载体、快速检测技术开发等工作,E-mail:348232911@qq.com
基金资助:
新疆维吾尔自治区重点研发计划（2019B00002）

Investigation of rodents and their ectoparasites and pathogens at Horgos port in Xinjiang, China, 2020

TIAN Feng¹, GENG He-yuan², XIAHOU Wei-min³, ZHANG Zi-long⁴, GUO Qiang⁵, ZHAO Dan-yun⁵, SUN Yu-ting¹, SHI Yan¹, CAI Xiao-xue¹, CHEN Chao-yi¹, JIANG Lu¹, CHENG Hui¹

1. Medical Examination Department, Xinjiang International Travel Health Care Center (Urumqi Customs Port Outpatient Department), Urumqi, Xinjiang 830011, China;
2. Jiangsu International Travel Healthcare Center (Nanjing Customs Port Outpatient Department), Nanjing, Jiangsu 210000, China;
3. Comprehensive Technical Service Center of Shaoguan Customs, Shaoguan, Guangdong 512023, China;
4. Shanghai International Travel Healthcare Center (Shanghai Customs Port Outpatient Department), Shanghai 200050, China;
5. Tianjin International Travel healthcare Center (Tianjin Customs Port Outpatient Deparment), Tianjin 300456, China

Received:2022-05-25 Online:2022-12-20 Published:2022-12-09
Supported by:
Key Research and Development Program of Xinjiang Uygur Autonomous Region (No. 2019B00002)

摘要/Abstract

摘要： 目的了解新疆维吾尔自治区霍尔果斯口岸地区鼠种类和携带病原体情况。方法 2020年，在霍尔果斯口岸地区采用夹日法和夹夜法捕鼠，对捕获鼠类及体表寄生虫进行形态鉴定，采集鼠类脾脏和肺组织，用PCR方法检测脾脏的嗜吞噬细胞无形体、莱姆病螺旋体、鼠疫耶尔森菌（鼠疫菌）、斑点热群立克次体，用反转录聚合酶链式反应（RT-PCR）方法检测肺组织的汉坦病毒核酸。结果 2020年，在霍尔果斯口岸地区捕鼠9种142只，优势鼠种为小林姬鼠和大沙鼠；体表寄生蜱类4种25只，优势种为全沟硬蜱；体表寄生蚤类8种203匹，优势种为臀突客蚤。鼠脾中检出嗜吞噬细胞无形体核酸阳性8株和斑点热群立克次体核酸阳性1株，未检测到鼠疫菌、汉坦病毒、莱姆病螺旋体。结论掌握了霍尔果斯口岸地区鼠类及体表寄生虫的构成以及携带病原体情况，结果对当地鼠、蜱、蚤及相关疾病防控有一定参考价值。

关键词: 鼠类, 体表寄生虫, 病原体

Abstract: Objective To investigate the rodent species and carried ectoparasites and pathogens at Horgos port in Xinjiang, China. Methods Rodents were captured by day snap-trapping and night snap-trapping methods at Horgos port in 2020. Morphological identification was carried out for the captured rodents and ectoparasites. The spleen and lung tissues of rodents were collected. Anaplasma phagocytophilum, Borrelia burgdorferi, Yersinia pestis, and spotted fever group rickettsiae in the spleen tissue were measured by polymerase chain reaction (PCR). Hantavirus nucleic acid in the lung tissue was measured by reverse transcription-PCR. Results In 2020, 142 rodents of 9 species were caught at Horgos port, and the dominant species were Apodemus sylvaticus and Rhombomys opimus. There were 25 parasitic ticks on the body surface belonging to 4 species, and the dominant species was Ixodes persulcatus. There were 203 parasitic fleas on the body surface belonging to 8 species, and the dominant species was Xenopsylla minax. Of the rodent ticks, 8 ticks tested positive for A. phagocytophilum nucleic acid, and 1 tick tested positive for spotted fever group rickettsiae nucleic acid; Y. pestis, hantavirus, and B. burgdorferi were not detected. Conclusion The composition of rodents and ectoparasites, as well as the carried pathogens at Horgos port provide some valuable reference for the prevention and control of rodents, ticks, fleas and related diseases in the local area.

Key words: Rodents, Ectoparasites, Pathogens

中图分类号:

田锋, 耿合员, 夏侯为民, 张子龙, 郭强, 赵丹云, 孙玉婷, 史燕, 蔡晓雪, 陈超怡, 蒋璐, 程慧. 2020年新疆霍尔果斯口岸地区鼠类及其体表寄生虫携带病原体调查[J]. 中国媒介生物学及控制杂志, 2022, 33(6): 906-911.

TIAN Feng, GENG He-yuan, XIAHOU Wei-min, ZHANG Zi-long, GUO Qiang, ZHAO Dan-yun, SUN Yu-ting, SHI Yan, CAI Xiao-xue, CHEN Chao-yi, JIANG Lu, CHENG Hui. Investigation of rodents and their ectoparasites and pathogens at Horgos port in Xinjiang, China, 2020[J]. Chinese Journal of Vector Biology and Control, 2022, 33(6): 906-911.

参考文献

[1] 靳木子,云华,陈宇飞,等. 2014-2015年内蒙古口岸地区鼠类及其携带病原体调查[J]. 中国媒介生物学及控制杂志,2017,28(4):347-349. DOI:10.11853/j.issn.1003.8280.2017. 04.010. Jin MZ,Yun H,Chen YF,et al. Investigation and analysis of rodent-borne pathogens in Inner Mongolia port areas of China from 2014 to 2015[J]. Chin J Vector Biol Control,2017,28(4):347-349. DOI:10.11853/j.issn.1003.8280.2017.04.010.(in Chinese)
[2] 靳木子,张朝富,韩丹,等. 内蒙古满洲里口岸地区2013-2015年鼠类病原体携带情况调查[J]. 中国媒介生物学及控制杂志,2017,28(2):173-174. DOI:10.11853/j.issn.1003.8280. 2017.02.020. Jin MZ,Zhang CF,Han D,et al. Investigation and analysis of rodent pathogen harborage in Inner Mongolia Manchuria port area during 2013-2015[J]. Chin J Vector Biol Control,2017,28(2):173-174. DOI:10.11853/j.issn.1003.8280.2017.02.020.(in Chinese)
[3] 郭强,田锋,卡布努尔·马木提,等. 2016年新疆霍尔果斯口岸地区鼠类及其携带病原体调查[J]. 中国国境卫生检疫杂志,2017,40(4):276-277,289. DOI:10.16408/j.1004-9770.2017. 04.012. Guo Q,Tian F,Kabnur MMT,et al. Investigation on rats and pathogens at Horgos port regions in Xinjiang,2016[J]. Chin J Front Health Quarant,2017,40(4):276-277,289. DOI:10. 16408/j.1004-9770.2017.04.012.(in Chinese)
[4] 尹小平,刘戈,郑常东,等. 中哈边境病媒生物及携带病原体入侵的监控探讨[J]. 中国媒介生物学及控制杂志,2019,30(4):458-463. DOI:10.11853/j.issn.1003.8280.2019.04.026. Yin XP,Liu G,Zheng CD,et al. Surveillance and control of vectors and related pathogens in the China-Kazakhstan border[J]. Chin J Vector Biol Control,2019,30(4):458-463. DOI:10.11853/j.issn.1003.8280.2019.04.026.(in Chinese)
[5] 中华人民共和国国家质量监督检验检疫总局. SN/T 4611-2016国境口岸斑点热群立克次体巢式PCR检测方法[S]. 北京:中国标准出版社,2017. General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China. SN/T 4611-2016 Nested PCR detecting method for spotted fever group rickettsiae at frontier port[S]. Beijing:Standards Press of China,2017. (in Chinese)
[6] Chu CY,Jiang BG,Liu W,et al. Presence of pathogenic Borrelia burgdorferi sensu lato in ticks and rodents in Zhejiang,south-east China[J]. J Med Microbiol,2008,57(8):980-985. DOI:10.1099/jmm.0.47663-0.
[7] Wen BH,Jian R,Zhang YZ,et al. Simultaneous detection of Anaplasma marginale and a new Ehrlichia species closely related to E. chaffeensis by sequence analyses of 16S ribosomal DNA in Boophilus microplus ticks from Tibet[J]. J Clin Microbiol,2002,40(9):3286-3290. DOI:10.1128/JCM.40.9. 3286-3290.2002.
[8] 中华人民共和国国家质量监督检验检疫总局. SN/T 4276-2015国境口岸鼠疫耶尔森菌荧光PCR检测方法[S]. 北京:中国标准出版社,2016. General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China. SN/T 4276-2015 Detection of Yersinia pestis in rodents by real-time PCR method at frontier port[S]. Beijing:Standards Press of China,2016. (in Chinese)
[9] 高玉峰,宋桂荣,谭泽,等. 2008-2015年辽宁口岸鼠类及其携带汉坦病毒的监测检测[J]. 中国国境卫生检疫杂志,2017,40(2):100-103. DOI:10.16408/j.1004-9770.2017.02.007. Gao YF,Song GR,Tan Z,et al. Analysis on population structure of rodents and Hantavirus infection at Liaoning ports from 2008 to 2015[J]. Chin J Front Health Quarant,2017,40(2):100-103. DOI:10.16408/j.1004-9770.2017.02.007.(in Chinese)
[10] 柴文丽,王宇平,张建明,等. 2010-2012年福建口岸鼠形动物携带病原体监测结果分析[J]. 中国国境卫生检疫杂志,2013,36(4):223-227. DOI:10.16408/j.1004-9770.2013.04.005. Chai WL,Wang YP,Zhang JM,et al. Analysis on pathogens monitoring results of rodent at Fujian port from 2010 to 2012[J]. Chin J Front Health Quarant,2013,36(4):223-227. DOI:10.16408/j.1004-9770.2013.04.005.(in Chinese)
[11] 尹小平,程晓兰,巴特尔,等. 首次从阿拉山口口岸地区沙鼠中检测到博卡病毒[J]. 中国国境卫生检疫杂志,2018,41(5):338-341. DOI:10.16408/j.1004-9770.2018.05.009. Yin XP,Cheng XL,Bateer,et al. The first detection of Bocavirus from gerbil at Alashankou border region[J]. Chin J Front Health Quarant,2018,41(5):338-341. DOI:10.16408/j.1004-9770. 2018.05.009.(in Chinese)
[12] 张庄熠,吴荣泉. 2014-2015年泉州港鼠类及其体表寄生虫调查[J]. 中国国境卫生检疫杂志,2016,39(4):265-268. DOI:10.16408/j.1004-9770.2016.04.011. Zhang ZY,Wu RQ. Survey on rats and ectoparasites at Quanzhou port,2014-2015[J]. Chin J Front Health Quarant,2016,39(4):265-268. DOI:10.16408/j.1004-9770.2016.04.011.(in Chinese)
[13] 阿不都热合曼·吐尔逊,余亮,艾尼瓦尔·吐米尔,等. 新疆北部干旱地区鼠类群落空间结构差异的比较研究[J]. 新疆大学学报(自然科学版),2008,25(2):211-218. DOI:10.3969/j.issn.1000-2839.2008.02.017. Tursunl A,Yu L,Tumur A,et al. Comparative study on the spatial structure differences of the rodent communities from arid areas in Northern Xinjiang[J]. J Xinjiang Univ (Nat Sci Ed),2008,25(2):211-218. DOI:10.3969/j.issn.1000-2839.2008. 02.017.(in Chinese)
[14] 郭宝平,郭刚,张莉,等. 新疆察布查尔县小型啮齿类动物多房棘球蚴感染情况调查[J]. 中国寄生虫学与寄生虫病杂志,2021,39(3):327-332. DOI:10.12140/j.issn.1000-7423.2021. 03.006. Guo BP,Guo G,Zhang L,et al. Investigation on infection of Echinococcus multilocularis metacestode in small rodents in Chabchar county,Xinjiang[J]. Chin J Parasitol Parasit Dis,2021,39(3):327-332. DOI:10.12140/j.issn.1000-7423.2021. 03.006.(in Chinese)
[15] 贵有军,史深,罗勇军,等. 新疆啮齿动物区系构成及地理分布[J]. 中华卫生杀虫药械,2021,27(6):557-564. DOI:10. 19821/j.1671-2781.2021.06.021. Gui YJ,Shi S,Luo YJ,et al. The fauna and geographical distribution of rodents in Xinjiang[J]. Chin J Health Insect Equip,2021,27(6):557-564. DOI:10.19821/J.1671-2781. 2021.06.021.(in Chinese)
[16] 王安东. 阿拉山口口岸常见媒介生物本底调查及携带病原谱检测研究[D]. 石河子:石河子大学,2016. Wang AD. Identification of vetor species and investigation of diseases in Alashankou port[D]. Shihezi:Shihezi University,2016. (in Chinese)
[17] 杨常青,郝艳阳,李树峰,等. 2019年内蒙古西部口岸地区蜱类及其携带病原体调查[J]. 中国国境卫生检疫杂志,2021,44(5):327-329. DOI:10.16408/j.1004-9770.2021.05.006. Yang CQ,Hao YY,Li SF,et al. Investigation of ticks and pathogens in the western ports of Inner Mongolia,2019[J]. Chin J Front Health Quarant,2021,44(5):327-329. DOI:10.16408/J.1004-9770.2021.05.006.(in Chinese)
[18] 曲志强,林汉亮,许肖枫,等. 新疆部分地区蜱传斑点热立克次体的分子流行病学研究[J]. 甘肃农业大学学报,2019,54(5):10-16. DOI:10.13432/j.cnki.jgsau.2019.05.002. Qu ZQ,Lin HL,Xu XF,et al. Molecular epidemiological study on the spotted fever rickettsia in Xinjiang[J]. J Gansu Agric Univ,2019,54(5):10-16. DOI:10.13432/j.cnki.jgsau.2019.05.002.(in Chinese)
[19] 王茜. 新疆蜱携病毒谱解析与斑点热感染病例分析[D]. 石河子:石河子大学,2018. Wang Q. Analysis of the virus of tick species and tick-borne Rickettsiae infection cases in Xinjiang[D]. Shihezi:Shihezi University,2018. (in Chinese)

2020年新疆霍尔果斯口岸地区鼠类及其体表寄生虫携带病原体调查

Investigation of rodents and their ectoparasites and pathogens at Horgos port in Xinjiang, China, 2020

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