口岸截获国内未见分布种小异甲蠊（Diplopterina parva）形态学与分子生物学鉴定

doi:10.11853/j.issn.1003.8280.2022.06.018

中国媒介生物学及控制杂志 ›› 2022, Vol. 33 ›› Issue (6): 859-864.DOI: 10.11853/j.issn.1003.8280.2022.06.018

口岸截获国内未见分布种小异甲蠊（Diplopterina parva）形态学与分子生物学鉴定

刘德星^1,2, 李婷婷^1,2, 魏晓雅^1,2, 岳巧云³, 邱德义², 柯明剑¹, 聂维忠⁴, 陈健^1,2

1. 珠海国际旅行卫生保健中心(拱北海关口岸门诊部)国家医学媒介生物检测重点实验室(广东), 广东珠海 519020;
2. 中山海关技术中心卫生检疫实验室, 广东中山 528400;
3. 中山火炬职业技术学院, 广东中山 528436;
4. 秦皇岛海关, 河北秦皇岛 066002

收稿日期:2022-05-24 出版日期:2022-12-20 发布日期:2022-12-09
通讯作者: 陈健,E-mail:chenjian5861111@163.com
作者简介:刘德星,男,工程师,从事病媒生物物种鉴定及其携带病原体研究,E-mail:596217742@qq.com;李婷婷,女,助理工程师,从事病媒生物物种鉴定及其携带病原体研究,E-mail:645537712@qq.com
基金资助:
海关总署科研项目（2020HK121，2021HK144，2021HK005）；中山市重大公益类项目（2021B2009）；拱北海关科研项目（2021GK009）；河北省重点研发计划（213777109D）

Morphological and molecular identification of a non-recorded cockroach species, Diplopterina parva, inceperated at a port of China

LIU De-xing^1,2, LI Ting-ting^1,2, WEI Xiao-ya^1,2, YUE Qiao-yun³, QIU De-yi², KE Ming-jian¹, NIE Wei-zhong⁴, CHEN Jian^1,2

1. State Key Laboratory of Medical Vector Surveillance, Zhuhai International Travel Healthcare Center, Zhuhai, Guangdong 519020, China;
2. Health Quarantine Laboratory, Zhongshan Customs Technology Center, Zhongshan, Guangdong 528400, China;
3. Zhongshan Torch Polytechnic, Zhongshan, Guangdong 528436, China;
4. Qinhuangdao Customs, Qinhuangdao, Hebei 066002, China

Received:2022-05-24 Online:2022-12-20 Published:2022-12-09
Supported by:
General Administration of Customs Research Projects (No. 2020HK121, 2021HK144, 2021HK005);Zhongshan City Major Public Welfare Projects (No. 2021B2009); Gongbei Customs Research Projects (No. 2021GK009); Key R&D Program of Hebei Province (No. 213777109D)

摘要/Abstract

摘要： 目的利用形态学与分子生物学的方法对中山港口岸进口集装箱中截获的2只蜚蠊进行种类鉴定。方法 2021年11月在中山港口岸来自尼日利亚的集装箱截获了1只雌性蜚蠊和1只蜚蠊若虫，通过形态学方法检索雌性蜚蠊的形态特征，并同时提取2只蜚蠊基因组DNA，用线粒体细胞色素C氧化酶亚基Ⅰ（COⅠ）的基因片段进行PCR扩增和测序，测序结果在GenBank中进行比对，采用邻接法构建系统进化树。结果雌性蜚蠊形态鉴定为小异甲蠊，2只蜚蠊的COⅠ基因序列在GenBank中未比对出相似度>98%的同源物种，但是2只蜚蠊COⅠ基因序列相互比对相似度>98%，可判定该蜚蠊若虫与雌性成虫同为小异甲蠊（Diplopterina parva）。经查证国内未见该种蜚蠊分布。结论中山港口岸进口集装箱截获的2只蜚蠊通过形态学和分子生物学的方法鉴定均为小异甲蠊，为国内未见分布种。

关键词: 国内未见分布种, 小异甲蠊, 形态学鉴定, 分子生物学鉴定, 中山口岸

Abstract: Objective To identify the species of two cockroaches intercepted in the import containers at Zhongshan port in China by morphological and molecular methods. Methods A female cockroach and a cockroach nymph were intercepted in the imported containers from Nigeria at Zhongshan port in November, 2021. The morphological characteristics of the female cockroach were retrieved by the morphological method, and the genomic DNA was extracted from the two cockroaches. The gene segment of mitochondrial cytochrome c oxidase subunit Ⅰ (COⅠ) was amplified by polymerase chain reaction and sequenced, and the sequencing results were compared in GenBank. The phylogenetic tree was constructed by neighbor-joining method. Results The female cockroach was morphologically identified as Diplopterina parva. The COⅠ gene sequences of the two cockroaches did not compare the homologous species with a similarity of more than 98% in GenBank, but the similarity of the COⅠ gene sequences of the two cockroaches was >98%. The cockroach nymph was identified as D. parva. It has been verified that no distribution of this species has been seen in China. Conclusion The two cockroaches intercepted in the imported containers at Zhongshan port are identified as D. parva by morphological and molecular methods, which is a non-recorded species in China.

Key words: Non-recorded specimens in China, Diplopterina parva, Morphology, Molecular identification, Zhongshan port

中图分类号:

R384.9

刘德星, 李婷婷, 魏晓雅, 岳巧云, 邱德义, 柯明剑, 聂维忠, 陈健. 口岸截获国内未见分布种小异甲蠊（Diplopterina parva）形态学与分子生物学鉴定[J]. 中国媒介生物学及控制杂志, 2022, 33(6): 859-864.

LIU De-xing, LI Ting-ting, WEI Xiao-ya, YUE Qiao-yun, QIU De-yi, KE Ming-jian, NIE Wei-zhong, CHEN Jian. Morphological and molecular identification of a non-recorded cockroach species, Diplopterina parva, inceperated at a port of China[J]. Chinese Journal of Vector Biology and Control, 2022, 33(6): 859-864.

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口岸截获国内未见分布种小异甲蠊（Diplopterina parva）形态学与分子生物学鉴定

Morphological and molecular identification of a non-recorded cockroach species, Diplopterina parva, inceperated at a port of China

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