蚊浓核病毒亚致死剂量感染对白纹伊蚊溴氰菊酯抗药性的影响研究

doi:10.11853/j.issn.1003.8280.2022.04.011

中国媒介生物学及控制杂志 ›› 2022, Vol. 33 ›› Issue (4): 499-502.DOI: 10.11853/j.issn.1003.8280.2022.04.011

蚊浓核病毒亚致死剂量感染对白纹伊蚊溴氰菊酯抗药性的影响研究

范苏云¹, 石向辉¹, 陈建¹, 陈建忠¹, 顾金保²

1. 深圳市福田区疾病预防控制中心传染病管理科, 广东深圳 518040;
2. 南方医科大学公共卫生学院, 广东广州 510515

收稿日期:2021-11-12 出版日期:2022-08-20 发布日期:2022-08-12
作者简介:范苏云,女,主任医师,主要从事病媒生物防制工作,E-mail:16722293@qq.com
基金资助:
深圳市科技项目（JCYJ20180302153626462）；国家自然科学基金（81371846）

Effect of sublethal dose of Mosquito densovirus infection on deltamethrin resistance of Aedes albopictus

FAN Su-yun¹, SHI Xiang-hui¹, CHEN Jian¹, CHEN Jian-zhong¹, GU Jin-bao²

1. Division of Infectious Disease Prevention and Control, Futian District Center for Disease Control and Prevention, Shenzhen, Guangdong 518040, China;
2. School of Public Health, Southern Medical University, Guangzhou, Guangdong 510515, China

Received:2021-11-12 Online:2022-08-20 Published:2022-08-12
Supported by:
Shenzhen Science and Technology Projects (No. JCYJ20180302153626462); National Natural Science Foundation of China (No. 81371846)

摘要/Abstract

摘要： 目的研究白纹伊蚊浓核病毒-7（AalDV-7）亚致死剂量感染对白纹伊蚊溴氰菊酯抗药性的影响。方法对实验室白纹伊蚊敏感品系施加溴氰菊酯进行筛选，建立抗性品系。利用亚致死剂量（10⁸ geq/ml）的AalDV-7感染白纹伊蚊幼虫，通过抗药性生物测定法检测感染组和未感染组幼蚊的半数致死浓度（LC₅₀）和成蚊死亡率，使用SPSS 19.0软件通过t检验与χ²检验分别比较幼蚊LC₅₀与成蚊死亡率。结果经15代筛选成功建立了实验室白纹伊蚊溴氰菊酯抗性品系。亚致死剂量AalDV-7感染后，幼蚊LC₅₀由0.030 0 mg/L降低至0.018 5 mg/L（t=12.521，P<0.001），降为中等抗性，成蚊抗药性生物测定法死亡率由65.7%提高到78.1%（χ²=5.079，P=0.015），但抗性水平未变。结论亚致死剂量AalDV-7感染可降低白纹伊蚊幼虫溴氰菊酯抗药性，在一定程度上起到了增效剂的作用，结果为解决蚊媒抗药性提供了新的理论依据与策略。

关键词: 白纹伊蚊, 白纹伊蚊浓核病毒, 溴氰菊酯抗药性

Abstract: Objective To study the effect of sublethal dose of Aedes albopictus densovirus-7 (AalDV-7) infection on the deltamethrin resistance of Ae. albopictus. Methods The laboratory resistant strain of Ae. albopictus was established by applying deltamethrin insecticide to the laboratory susceptible strain. Ae. albopictus larvae were infected with a sublethal dose of AalDV-7 (10⁸ geq/ml). The half-lethal concentration (LC₅₀) for larvae and the biological mortality of adult mosquitos in the infected and uninfected groups were determined using the resistance bioassay, and were compared by the t test and χ² test using the SPSS 19.0 software, respectively. Results The laboratory strain of Ae. albopictus resistant to deltamethrin was successfully established after 15 consecutive generations of screening. After sublethal dose of AalDV-7 infection, the LC₅₀ for larvae decreased from 0.030 0 mg/L to 0.018 5 mg/L (moderate resistance) (t=12.521, P<0.001), and the biological mortality of adult mosquitoes increased from 65.7% to 78.1% (χ²=5.079, P=0.015), with the resistance level unchanged. Conclusion Sublethal dose of AalDV-7 infection reduces the deltamethrin resistance of Ae. albopictus larvae and acts as a synergist to some extent, providing a new theoretical basis and strategy for solving mosquito resistance.

Key words: Aedes albopictus, Aedes albopictus densovirus, Deltamethrin resistance

中图分类号:

R384.1

范苏云, 石向辉, 陈建, 陈建忠, 顾金保. 蚊浓核病毒亚致死剂量感染对白纹伊蚊溴氰菊酯抗药性的影响研究[J]. 中国媒介生物学及控制杂志, 2022, 33(4): 499-502.

FAN Su-yun, SHI Xiang-hui, CHEN Jian, CHEN Jian-zhong, GU Jin-bao. Effect of sublethal dose of Mosquito densovirus infection on deltamethrin resistance of Aedes albopictus[J]. Chinese Journal of Vector Biology and Control, 2022, 33(4): 499-502.

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蚊浓核病毒亚致死剂量感染对白纹伊蚊溴氰菊酯抗药性的影响研究

Effect of sublethal dose of Mosquito densovirus infection on deltamethrin resistance of Aedes albopictus

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