中国媒介生物学及控制杂志 ›› 2021, Vol. 32 ›› Issue (2): 221-223.DOI: 10.11853/j.issn.1003.8280.2021.02.020

• 分类鉴定 • 上一篇    下一篇

1株分离于青海省喜马拉雅旱獭的布鲁氏菌微量生化鉴定

薛红梅, 谢辉, 马丽, 张爱萍, 于守鸿, 李积权, 杨旭欣, 徐立青   

  1. 青海省地方病预防控制所布鲁氏菌病预防控制科, 青海 西宁 810021
  • 收稿日期:2020-05-12 出版日期:2021-04-20 发布日期:2021-04-20
  • 通讯作者: 徐立青,E-mail:qhxlq2006@163.com
  • 作者简介:薛红梅,女,硕士,主管医师,主要从事布病防治工作,E-mail:qhxhm2009@126.com
  • 基金资助:
    国家自然科学基金(81860588)

Microbiochemical identification of a Brucella strain isolated from Marmota himalayana in Qinghai province, China

XUE Hong-mei, XIE Hui, MA Li, ZHANG Ai-ping, YU Shou-hong, LI Ji-quan, YANG Xu-xin, XU Li-qing   

  1. Department of Brucellosis Prevention and Control, Qinghai Provincial Institute of Endemic Disease Prevention and Control, Xining, Qinghai 810021, China
  • Received:2020-05-12 Online:2021-04-20 Published:2021-04-20
  • Supported by:
    Supported by the National Natural Science Foundation of China (No. 81860588)

摘要: 目的 对2017年自青海省喜马拉雅旱獭组织中分离出的1株疑似布鲁氏菌菌株进行生化鉴定。方法 采用细菌微量生化管和常规病原学鉴定,对分离出的菌株进行检测,同时与3种布鲁氏菌标准菌株的检测结果进行对比。结果 微量生化鉴定显示,该菌株可氧化尿素,不氧化鸟氨酸、赖氨酸、D-半乳糖、山梨醇、木糖、葡萄糖酸盐,为羊种布鲁氏菌。结论 细菌微量生化鉴定方法快速简单,可为布鲁氏菌检验提供一个快速的辅助实验依据。

关键词: 喜马拉雅旱獭, 分离菌株, 微量生化鉴定, 青海省

Abstract: Objective To perform microbiochemical identification of a suspected Brucella strain isolated from Marmota himalayana in Qinghai province, China in 2017. Methods The isolated bacteria strains were detected by microbiochemical tube and routine pathogenic identification, and the results were compared with the results of three standard Brucella strains. Results Microbiochemical identification showed that the strain could oxidize urea and did not oxidize ornithine, lysine, D-galactose, sorbitol, xylose, and gluconate. Therefore, it was identified as Brucella melitensis. Conclusion Microbiochemical identification of bacteria is rapid and simple, and can provide an experimental basis for the detection of Brucella.

Key words: Marmota himalayana, Isolated bacteria strain, Microbiochemical identification, Qinghai province

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