海南岛中部山区鼠类肠道菌群多样性研究

doi:10.11853/j.issn.1003.8280.2020.05.019

中国媒介生物学及控制杂志 ›› 2020, Vol. 31 ›› Issue (5): 596-601.DOI: 10.11853/j.issn.1003.8280.2020.05.019

海南岛中部山区鼠类肠道菌群多样性研究

周洁¹, 周晓君¹, 轩断断¹, 潘良源¹, 梁佳欣¹, 陈宗亮¹, 张萌萌¹, 苏容玉¹, 边晨缤¹, 董雨欣¹, 胡守奎², 牛莉娜¹

1 海南医学院基础医学与生命科学学院, 海南海口 571199;
2 北京大学首钢医院, 北京 100144

收稿日期:2020-04-23 出版日期:2020-10-20 发布日期:2020-10-20
通讯作者: 牛莉娜,Email:66205894@qq.com
作者简介:周洁,女,在读本科,主要从事病原生物学研究工作,Email:1501364909@qq.com
基金资助:
2019年度海南省高等学校科学研究项目（Hnky2019ZD-27）；国家自然科学基金（81760376）；2018年国家级大学生创新创业训练计划项目（20181181024）；海南医学院2018年大学生创新创业训练计划项目（HYCX2018024）；2019年海南省大学生创新创业训练计划项目（S201911810034）

Study on the diversity of intestinal microbiota of wild rodents from the central mountainous area of Hainan Island, China

ZHOU Jie¹, ZHOU Xiao-jun¹, XUAN Duan-duan¹, PAN Liang-yuan¹, LIANG Jia-xin¹, CHEN Zong-liang¹, ZHANG Meng-meng¹, SU Rong-yu¹, BIAN Chen-bin¹, DONG Yu-xin¹, HU Shou-kui², NIU Li-na¹

1 Hainan Medical College, Haikou 571199, Hainan Province, China;
2 Peking University Shougang Hospital

Received:2020-04-23 Online:2020-10-20 Published:2020-10-20
Supported by:
Supported by the Project Funding of Education Department of Hainan Province in 2019 (No. Hnky2019ZD-27), National Natural Science Foundation of China (No. 81760376), National Innovation and Entrepreneurship Training Program for College Students in 2018 (No. 20181181024), Innovative Experimental Project of Hainan Medical College Students in 2018 (No. HYCX2018024) and Innovation and Entrepreneurship Training Program for College Students in Hainan Province in 2019 (No. S201911810034)

摘要/Abstract

摘要： 目的研究海南岛中部山区鼠类肠道菌群结构的多样性。方法采用高通量测序技术分析野生鼠类肠道粪便样本菌群的结构组成并进行多样性分析。结果从20只鼠类粪便样本中共获得1 569 543条有效序列，操作分类单元范围为540~1 274。门分类水平上厚壁菌门所占比例最高（55.83%），拟杆菌门为第二大优势菌门（27.74%）。属分类水平位于前10位的是螺杆菌属、乳杆菌属、梭菌属、大肠埃希菌-志贺菌属、链球菌属、毛螺菌属、粪杆菌属、梭杆菌属、支原体和Romboutsia。结论高通量测序分析发现海南岛中部山区鼠类肠道菌群结构复杂，菌种丰富，个体间存在较大差异，有6个样本螺杆菌属相对丰度最高，有进一步研究的意义和价值。

关键词: 鼠类, 高通量测序, 肠道菌群多样性

Abstract: Objective To investigate the diversity of intestinal microbiota of wild rodents from the central mountainous area of Hainan Island, China. Methods The high-throughput sequencing technology was used to sequence the DNA extracted from fecal samples of wild rodents, and the obtained sequence data were analyzed using bioinformatic methods to investigate the composition and diversity of intestinal microbiota. Results A total of 1 569 543 valid sequences were obtained from the fecal samples of 20 wild rodents. The number of operational taxonomic units ranged from 540 to 1 274. At the phylum level, the main flora of the studied wild rodents was Firmicutes (55.83%), followed by Bacteroidetes (27.74%). At the genus level, the top 10 bacterial genera were Helicobacter, Lactobacillus, Clostridium, Escherichia-Shigella, Streptococcus, Lachnospiraceae, Faecalitalea, Fusobacterium, Mycoplasma, and Romboutsia. Conclusion High-throughput sequencing analysis revealed that the structure of the intestinal microbiota of wild rodents in the central mountainous area of Hainan Island was complex, with abundant species and large differences among individuals. Helicobacter had the highest relative abundance in six fecal samples, which was of significance and value for further investigations.

Key words: Wild rodents, High-throughput sequencing, Intestinal microbiota diversity

中图分类号:

R378
R184

周洁, 周晓君, 轩断断, 潘良源, 梁佳欣, 陈宗亮, 张萌萌, 苏容玉, 边晨缤, 董雨欣, 胡守奎, 牛莉娜. 海南岛中部山区鼠类肠道菌群多样性研究[J]. 中国媒介生物学及控制杂志, 2020, 31(5): 596-601.

ZHOU Jie, ZHOU Xiao-jun, XUAN Duan-duan, PAN Liang-yuan, LIANG Jia-xin, CHEN Zong-liang, ZHANG Meng-meng, SU Rong-yu, BIAN Chen-bin, DONG Yu-xin, HU Shou-kui, NIU Li-na. Study on the diversity of intestinal microbiota of wild rodents from the central mountainous area of Hainan Island, China[J]. Chines Journal of Vector Biology and Control, 2020, 31(5): 596-601.

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海南岛中部山区鼠类肠道菌群多样性研究

Study on the diversity of intestinal microbiota of wild rodents from the central mountainous area of Hainan Island, China

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