RNA干扰技术在蚊虫防治工作中的研究进展

doi:10.11853/j.issn.1003.8280.2020.02.025

摘要/Abstract

摘要： RNA干扰技术自从被发现后，经过了20年的验证和发展，业已成为研究基因功能、大规模基因筛选等最主要的研究方法。近年来，RNA干扰技术在媒介蚊虫治理中不断得到应用，且取得一系列的成果。该文就RNA干扰作用机制、导入方法以及其在蚊虫防治工作中的研究进展进行综述。

关键词: RNA干扰, 蚊虫防治, 抗药性

Abstract: RNA interference technology, since its discovery in the late 1990s, has become the most important research method for studying gene function and large-scale gene screening after two decades of verification and development. In recent years, RNA interference has been constantly applied in vector mosquito control, and a series of achievements has been made. In this paper, the mechanism and introducing methods of RNA interference, as well as its research progress in mosquito prevention and control, were reviewed.

Key words: RNA interference, Mosquito prevention and control, Insecticide resistance

中图分类号:

R384.1
Q965

王海洋, 王洋, 宋晓, 程鹏, 公茂庆. RNA干扰技术在蚊虫防治工作中的研究进展[J]. 中国媒介生物学及控制杂志, 2020, 31(2): 234-238.

WANG Hai-yang, WANG Yang, SONG Xiao, CHENG Peng, GONG Mao-qing. Research progress in RNA interference technology in mosquito prevention and control[J]. Chines Journal of Vector Biology and Control, 2020, 31(2): 234-238.

参考文献

[1] Fire A,Xu SQ,Montgomery MK,et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans[J]. Nature,1998,391(6669):806-811. DOI:10.1038/35888.
[2] Campbell CL,Black IV WC,Hess AM,et al. Comparative genomics of small RNA regulatory pathway components in vector mosquitoes[J]. BMC Genomics,2008,9:425. DOI:10.1186/1471-2164-9-425.
[3] Pham JW,Pellino JL,Lee YS,et al. A dicer-2-dependent 80s complex cleaves targeted mRNAs during RNAi in Drosophila[J]. Cell,2004,117(1):83-94. DOI:10.1016/S0092-8674(04)00258-2.
[4] Schwarz DS,Hutvágner G,Du TT,et al. Asymmetry in the assembly of the RNAi enzyme complex[J]. Cell,2003,115(2):199-208. DOI:10.1016/S0092-8674(03)00759-1.
[5] Killiny N,Kishk A. Delivery of dsRNA through topical feeding for RNA interference in the citrus sap piercing-sucking hemipteran,Diaphorina citri[J]. Arch Insect Biochem Physiol,2017,95(2):e21394. DOI:10.1002/arch.21394.
[6] Huvenne H,Smagghe G. Mechanisms of dsRNA uptake in insects and potential of RNAi for pest control:a review[J]. J Insect Physiol,2010,56(3):227-235. DOI:10.1016/j.jinsphys. 2009.10.004.
[7] Yu XD,Killiny N. RNA interference of two glutathione S-transferase genes,Diaphorina citri DcGSTe2 and DcGSTd1,increases the susceptibility of Asian citrus psyllid (Hemiptera:Liviidae) to the pesticides fenpropathrin and thiamethoxam[J]. Pest Manag Sci,2018,74(3):638-647. DOI:10.1002/ps.4747.
[8] Takahashi T,Hamada A,Miyawaki K,et al. Systemic RNA interference for the study of learning and memory in an insect[J]. J Neurosci Methods,2009,179(1):9-15. DOI:10.1016/j.jneumeth.2009.01.002.
[9] Prentice K,Christiaens O,Pertry I,et al. RNAi-based gene silencing through dsRNA injection or ingestion against the African sweet potato weevil Cylas puncticollis (Coleoptera:Brentidae)[J]. Pest Manag Sci,2017,73(1):44-52. DOI:10.1002/ps.4337.
[10] Bona ACD,Chitolina RF,Fermino ML,et al. Larval application of sodium channel homologous dsRNA restores pyrethroid insecticide susceptibility in a resistant adult mosquito population[J]. Parasit Vectors,2016,9:397. DOI:10.1186/s13071-016-1634-y.
[11] Airs PM,Bartholomay LC. RNA Interference for mosquito and mosquito-borne disease control[J]. Insects,2017,8(1):4. DOI:10.3390/insects8010004.
[12] Balakrishna Pillai A,Nagarajan U,Mitra A,et al. RNA interference in mosquito:understanding immune responses,double-stranded RNA delivery systems and potential applications in vector control[J]. Insect Mol Biol,2017,26(2):127-139. DOI:10.1111/imb.12282.
[13] Li T,Cao CW,Yang T,et al. A G-protein-coupled receptor regulation pathway in cytochrome P450-mediated permethrin-resistance in mosquitoes,Culex quinquefasciatus[J]. Sci Rep,2015,5:17772. DOI:10.1038/srep17772.
[14] Lumjuan N,Rajatileka S,Changsom D,et al. The role of the Aedes aegypti Epsilon glutathione transferases in conferring resistance to DDT and pyrethroid insecticides[J]. Insect Biochem Mol Biol,2011,41(3):203-209. DOI:10.1016/j.ibmb. 2010.12.005.
[15] Silva APB,Santos JMM,Martins AJ. Mutations in the voltage-gated sodium channel gene of anophelines and their association with resistance to pyrethroids-a review[J]. Parasit Vectors,2014,7:450. DOI:10.1186/1756-3305-7-450.
[16] Davies TE,O'Reilly AO,Field LM,et al. Knockdown resistance to DDT and pyrethroids:from target-site mutations to molecular modelling[J]. Pest Manag Sci,2008,64(11):1126-1130. DOI:10.1002/ps.1617.
[17] Weetman D,Wilding CS,Neafsey DE,et al. Candidate-gene based GWAS identifies reproducible DNA markers for metabolic pyrethroid resistance from standing genetic variation in East African Anopheles gambiae[J]. Sci Rep,2018,8(1):2920. DOI:10.1038/s41598-018-21265-5.
[18] Hoa NT,Keene KM,Olson KE,et al. Characterization of RNA interference in an Anopheles gambiae cell line[J]. Insect Biochem Mol Biol,2003,33(9):949-957. DOI:10.1016/S0965-1748(03)00101-2.
[19] Vadivalagan C,Karthika P,Murugan K,et al. Genetic deviation in geographically close populations of the dengue vector Aedes aegypti (Diptera:Culicidae):influence of environmental barriers in South India[J]. Parasitol Res,2016,115(3):1149-1160. DOI:10.1007/s00436-015-4847-7.
[20] Shi QQ,Song X,Lv YY,et al. Potential risks associated with Japanese encephalitis prevalence in Shandong province,China[J]. Vector Borne Zoonotic Dis,2019,19(8):640-645. DOI:10.1089/vbz.2018.2416.
[21] Galiana-Arnoux D,Dostert C,Schneemann A,et al. Essential function in vivo for Dicer-2 in host defense against RNA viruses in drosophila[J]. Nat Immunol,2006,7(6):590-597. DOI:10.1038/ni1335.
[22] Salda? a MA,Etebari K,Hart CE,et al. Zika virus alters the microRNA expression profile and elicits an RNAi response in Aedes aegypti mosquitoes[J]. PLoS Negl Trop Dis,2017,11(7):e0005760. DOI:10.1371/journal.pntd.0005760.
[23] Blair CD. Mosquito RNAi is the major innate immune pathway controlling arbovirus infection and transmission[J]. Future Microbiol,2011,6(3):265-277. DOI:10.2217/fmb.11.11.
[24] Sánchez-Vargas I,Scott JC,Poole-Smith BK,et al. Dengue virus type 2 infections of Aedes aegypti are modulated by the mosquito's RNA interference pathway[J]. PLoS Pathog,2009,5(2),e1000299. DOI:10.1371/journal.ppat.1000299.
[25] Sánchez-Vargas I,Scott JC,Poole-Smith BK,et al. Dengue virus type 2 infections of Aedes aegypti are modulated by the mosquito's RNA interference pathway[J]. PLoS Pathog,2009,5(2):e1000299. DOI:10.1371/journal.ppat.1000299.
[26] Franz AWE,Sanchez-Vargas I,Adelman ZN,et al. Engineering RNA interference-based resistance to dengue virus type 2 in genetically modified Aedes aegypti[J]. Proc Natl Acad Sci USA,2006,103(11):4198-4203. DOI:10.1073/pnas.0600479103.
[27] Yan Z,Hu HY,Jiang X,et al. Widespread expression of piRNA-like molecules in somatic tissues[J]. Nucleic Acids Res,2011,39(15):6596-6607. DOI:10.1093/nar/gkr298.
[28] Varjak M,Maringer K,Watson M,et al. Aedes aegypti Piwi4 is a noncanonical PIWI protein involved in antiviral responses[J]. mSphere,2017,2(3):e00144-17. DOI:10.1128/mSphere.00144-17.
[29] Azrag RS,Ibrahim K,Malcolm C,et al. Laboratory rearing of Anopheles arabiensis:impact on genetic variability and implications for Sterile Insect Technique (SIT) based mosquito control in northern Sudan[J]. Malar J,2016,15(1):432. DOI:10.1186/s12936-016-1484-2.
[30] Whyard S,Erdelyan CN,Partridge AL,et al. Silencing the buzz:a new approach to population suppression of mosquitoes by feeding larvae double-stranded RNAs[J]. Parasit Vectors,2015,8:96. DOI:10.1186/s13071-015-0716-6.
[31] Carpenter VK,Drake LL,Aguirre SE,et al. SLC7 amino acid transporters of the yellow fever mosquito Aedes aegypti and their role in fat body TOR signaling and reproduction[J]. J Insect Physiol,2012,58(4):513-522. DOI:10.1016/j.jinsphys.2012. 01.005.
[32] Moussian B. Recent advances in understanding mechanisms of insect cuticle differentiation[J]. Insect Biochem Mol Biol,2010,40(5):363-375. DOI:10.1016/j.ibmb.2010.03.003.
[33] Liao CH,Upadhyay A,Liang J,et al. 3,4-Dihydroxyphenylacetaldehyde synthase and cuticle formation in insects[J]. Dev Comp Immunol,2018,83:44-50. DOI:10.1016/j.dci.2017.11.007.
[34] Chen J,Lu HR,Zhang L,et al. RNA interference-mediated knockdown of 3,4-dihydroxyphenylacetaldehyde synthase affects larval development and adult survival in the mosquito Aedes aegypti[J]. Parasit Vectors,2019,12(1):311. DOI:10.1186/s13071-019-3568-7.
[35] Elleuch J,Zribi Zghal R,Lacoix MN,et al. Evidence of two mechanisms involved in Bacillus thuringiensis israelensis decreased toxicity against mosquito larvae:genome dynamic and toxins stability[J]. Microbiol Res,2015,176:48-54. DOI:10.1016/j.micres.2015.04.007.
[36] Ben-Dov E. Bacillus thuringiensis subsp. israelensis and its dipteran-specific toxins[J]. Toxins (Basel),2014,6(4):1222-1243. DOI:10.3390/toxins6041222.
[37] Ni M,Ma W,Wang XF,et al. Next-generation transgenic cotton:pyramiding RNAi and Bt counters insect resistance[J]. Plant Biotechnol J,2017,15(9):1204-1213. DOI:10.1111/pbi. 12709.
[38] Lim ZX,Robinson KE,Jain RG,et al. Diet-delivered RNAi in Helicoverpa armigera-progresses and challenges[J]. J Insect Physiol,2016,85:86-93. DOI:10.1016/j.jinsphys.2015.11.005.
[39] Terradas G,Joubert DA,McGraw EA. The RNAi pathway plays a small part in Wolbachia-mediated blocking of dengue virus in mosquito cells[J]. Sci Rep,2017,7:43847. DOI:10.1038/srep43847.
[40] Khan AA,Betel D,Miller ML,et al. Transfection of small RNAs globally perturbs gene regulation by endogenous microRNAs[J]. Nat Biotechnol,2009,27(6):549-555. DOI:10.1038/nbt.1543.
[41] Lundgren JG,Duan JJ. RNAi-based insecticidal crops:potential effects on nontarget species[J]. BioScience,2013,63(8):657-665. DOI:10.1525/bio.2013.63.8.8.
[42] 王锦达. 赤拟谷盗RNAi及dsRNA脱靶效应的研究[D]. 南京:南京农业大学,2015. Wang JD. RNAi in Tribolium castaneum and the off target effect of dsRNA[D]. Nanjing:Nanjing Agricultural University,2015.
[43] Garbutt JS,Belles X,Richards EH,et al. Persistence of double-s tranded RNA in insect hemolymph as a potential determiner of RNA interference success:evidence from Manduca sexta and Blattella germanica[J]. J Insect Physiol,2013,59(2):171-178. DOI:10.1016/j.jinsphys.2012.05.013.
[44] Hakim RS,Baldwin K,Smagghe G. Regulation of midgut growth,development,and metamorphosis[J]. Annu Rev Entomol,2010,55(1):593-608. DOI:10.1146/annurev-ento-112408-085450.
[45] Bolognesi R,Ramaseshadri P,Anderson J,et al. Characterizing the mechanism of action of double-stranded RNA activity against western corn rootworm (Diabrotica virgifera virgifera LeConte)[J]. PLoS One,2012,7(10):e47534. DOI:10.1371/journal.pone.0047534.
[46] Liu JB,Wang RY,Ma DJ,et al. Branch-PCR constructed stable shRNA transcription nanoparticles have long-lasting RNAi effect[J]. Chem Bio Chem,2016,17(11):1038-1042. DOI:10.1002/cbic.201600047.
[47] Joga MR,Zotti MJ,Smagghe G,et al. RNAi efficiency,systemic properties,and novel delivery methods for pest insect control:what we know so far[J]. Front Physiol,2016,7:553. DOI:10.3389/fphys.2016.00553.