中国媒介生物学及控制杂志 ›› 2020, Vol. 31 ›› Issue (2): 133-136.DOI: 10.11853/j.issn.1003.8280.2020.02.003

所属专题: 抗药性监测专题

• 抗药性监测专题 • 上一篇    下一篇

我国不同地理种群白纹伊蚊乙酰胆碱酯酶基因分布调查

朱彩英1, 赵春春1, 刘起勇1, 李贵昌1, 闫冬明1,2, 宋秀平1, 王君1, 孟凤霞1   

  1. 1 中国疾病预防控制中心传染病预防控制所媒介生物控制室, 传染病预防控制国家重点实验室, 世界卫生组织媒介生物监测与管理合作中心, 北京 102206;
    2山东第一医科大学公共卫生学院, 山东 泰安 271016
  • 收稿日期:2020-03-03 出版日期:2020-04-20 发布日期:2020-04-20
  • 通讯作者: 孟凤霞,Email:mengfengxia@icdc.cn
  • 作者简介:朱彩英,女,在读硕士,主要从事蚊虫抗性分子监测研究,Email:zhucaiying_ivy@163.com
  • 基金资助:
    国家科技重大专项(2017ZX10303404002005,2018ZX10101002-002)

Distribution of the ace gene of Aedes albopictus in different geographical populations in China

ZHU Cai-ying1, ZHAO Chun-chun1, LIU Qi-yong1, LI Gui-chang1, YAN Dong-ming1,2, SONG Xiu-ping1, WANG Jun1, MENG Feng-xia1   

  1. 1 Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Vector Surveillance and Management, Beijing 102206, China;
    2 School of Public Health, Shandong First Medical University
  • Received:2020-03-03 Online:2020-04-20 Published:2020-04-20
  • Supported by:
    Supported by the National Science and Technology Major Project of China (No. 2017ZX10303404002005, 2018ZX10101002-002)

摘要: 目的 了解登革热媒介白纹伊蚊野外种群的乙酰胆碱酯酶(ace)基因在不同区域的基因型及分布特点,从而进行白纹伊蚊对有机磷和氨基甲酸酯类杀虫剂抗药性机制的初步探索。方法 2018年6-9月在海南省海口市、广东省广州市、云南省景洪市和贵州省兴义市的14个采样点(地理种群),共采集白纹伊蚊幼虫、蛹和成蚊422只,经形态学或分子生物学方法鉴定后,用无水乙醇浸泡,并于-20℃冻存备用。提取单只蚊虫的DNA,PCR扩增ace基因部分片段,测序后分析ace基因G119位点突变情况。结果 针对上述4个省的14个种群白纹伊蚊ace基因测定,发现白纹伊蚊ace基因G119位点仅存在个别碱基突变,并未发现氨基酸突变。共检测到2种等位基因,即野生型GGA/G(832/98.58%)和突变型GGG/G(12/1.42%);1种基因型,即野生型纯合子G/G(422/100%)。结论 仅有少部分白纹伊蚊个体ace基因G119位点发生了碱基突变,但该碱基替换并未引起相应的氨基酸突变。因此,白纹伊蚊对有机磷和氨基甲酸酯类杀虫剂的靶标抗药性机制亟需进一步探索。

关键词: 白纹伊蚊, 抗药性, 乙酰胆碱脂酶基因, 基因突变

Abstract: Objective To investigate the genotypes and distribution characteristics of the ace gene of the dengue vector Aedes albopictus (field population) in different regions of China, and to preliminarily explore their resistance mechanism to organophosphorus and carbamate insecticides. Methods A total of 422 larvae, pupae, or adults of Ae. albopictus were collected at 14 sampling sites (14 geographical populations) in Haikou of Hainan province, Guangzhou of Guangdong province, Jinghong of Yunnan province, and Xingyi of Guizhou province from June to September 2018, and they were identified by morphology or molecular biology, soaked in anhydrous ethanol, and stored at -20℃. DNA was extracted from individual mosquito, and the partial fragment of the ace gene was amplified by PCR, sequenced, and analyzed for mutation at G119. Results There were only a few base mutations but no amino acid mutations at G119 of the ace gene. Two alleles were detected, namely wild-type GGA/G (832/98.58%) and mutant-type GGG/G (12/1.42%), and one genotype was detected, namely wild-type homozygote G/G (422/100%). Conclusion Only a few individuals of Ae. albopictus showed a base mutation at G119 of the ace gene, but the base substitution did not cause the corresponding amino acid mutation. Therefore, the resistance mechanism of Ae. albopictus to organophosphorus and carbamate insecticides needs to be further explored.

Key words: Aedes albopictus, Insecticide resistance, Ace gene, Gene mutation

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